Peptide Antibody Services
Immunizing with a peptide corresponding to a specific region of a protein antigen is a popular method to develop custom antibodies. With this strategy, you only need the amino acid sequence of a predicted/selected epitope within the protein antigen, avoiding homologous regions to minimize cross reactivity with other proteins.
Antigenic peptides of 10-20 amino acids in length provide optimal epitope diversity while still offering superior specificity compared to immunizing with the full length protein sequence. The peptide is usually conjugated to a carrier protein such as KLH or BSA to enhance stimulation of an immune response. Antibodies are generated against the peptide sequence and the carrier protein. Therefore, the highly specific antibodies directed against the peptide are separated by affinity purification.
Why Use Peptide Antigens for Immunization?
- Chemical synthesis yields defined epitopes
- Low probability of cross-reactivity
- High flexibility in antigen selection
- Sequence diversity and PTMs can be addressed
- Easy to synthesize, cost-effective and stable
Applications for Antibody Services
- Antigen design
- Production of antigen conjugates
- Titer determination
- Hybridoma screening
- Epitope mapping
- Blocking peptides
JPT's Antibody Peptide Formats
Immunogen Design
- Design and production of immunogenic peptides (e.g. KLH, BSA, OVA conjugates or MAP –multiple antigenic peptides)
- Modified peptides carrying PTMs such as phosphorylation, citrullination, acetylation and more
- Design supported by data mining, modeling and bioinformatics
Assay Development
- Screening tools such as pre-coated peptide ELISA plates for fast and easy titer determination without the need of carrier conjugation
- B-Cell ELISpot (total IgG and peptide-specific)
- Identification of soluble biomarkers as target for antibody development
Antibody Development
- Sophisticated peptide microarrays improve and accelerate your hybridoma screening
- Antibody epitope mapping service using our proprietary PepStar™ peptide microarray technology
- Specificity testing of antibodies (including PTMs)
- SAR for antibody/peptide interactions
- Design and production of mimotopes and biosimilars
Process Optimization
- Peptides as assay control
- Affinity Purification