Proteomics

JPT provides a multitude of innovative research tools to the proteomics community. Besides high -throughput chemical and analytical approaches to accelerate discovery of novel biomarkers from biological samples, JPT developed a new mass spectrometry-based peptide standard technique for absolute quantification of target proteins in clinical and targeted proteomics. Furthermore, JPT has a decade-long track record providing services and project expertise in the area of functional proteomics ranging from elucidation of protein-protein interactions and enzymatic activities to epigenetic regulation.

Mass Spectrometry-Based Proteomics

Functional Proteomics

Applications

Target discovery and validation
Biomarker discovery: we quantify hundreds of proteins, e.g. tumor associated antigens from patients’ blood samples, and correlate the quantities and post-translational modification status with clinical data
Clinical monitoring: monitoring the effects of therapeutic intervention and disease status are vital to understand and describe the effects of applied drugs on target proteins; especially the parallel quantification of large numbers of relevant proteins will facilitate personalized medicine approaches, patient stratification, and companion diagnostic development
Proteome wide quantification of proteins and protein modification states
Proteome wide characterization of enzymatic activities or enzyme families

Benefits

Proprietary high throughput peptide technologies for highly multiplexed identification of proteins
Unique approach for absolute and multiplexed quantification of proteins
Coverage of post-translational modifications
Rapid access to hundreds of thousands of peptides covering the entire human proteome

References

Proteome-wide Prediction of Peptide Tandem Mass Spectra by Deep Learning
Gessulat et al, Nature Methods (2019)
Development of Targeted Mass Spectrometry-Based Approaches for Quantitation of Proteins Enriched in the Postsynaptic Density (PSD)
Wilson et al, Proteomes (2019)
A Deep Proteome and Transcriptome Abundance Atlas of 29 Healthy Human Tissues
Wang et al, Molecular Systems Biology (2019)
Mining the Human Tissue Proteome for Protein Citrullination
Lee et al, Molecular & Cellular Proteomics (2018)
ProteomeTools: Systematic Characterization of 21 Post-Translational Protein Modifications by LC-MS/MS Using Synthetic Peptides
Zolg et al, Molecular & Cellular Proteomics (2018)
Analysis of Histone Modifications by Mass Spectrometry
Völker‐Albert et al, Current Protocols in Protein Science (2018)