What is SPOT Synthesis?
The SPOT peptide synthesis technique is an ultra-high-throughput synthesis method for hundreds to thousands of peptides in parallel. Peptides are synthesized on a coated cellulose membrane that is used as solid phase. After synthesis peptides can remain on the membrane for an easy and inexpensive peptide array (PepSpots) or be separated from the mebrane and used as individual peptides (PepTrack Fast Track), pooled (SpotMix) or used to print on glass slides (PepStar).
This automated process results in the production of huge numbers of peptides making this a fast and economical alternative to other methods that aim for deep T- and B-cell epitope discovery, MS-based proteomics relying on heavy & light reference peptides, antigen target identification and others.
Our SPOT peptides come in many ready to use formats, which allow a broad application range for well-known experimental procedures with a robust readout.
Our High Throughput Peptide Formats
| SpotMix (PLUS) Antigen Discovery Pools | PepStar (HD or Multiwell) Peptide Microarrays | PepSpots Peptide Arrays | PepTrack Fast Track (PLUS) Peptide Libraries | Peptide ELISA | BioTides Biotinylated Peptides | |
| Link to product page |  |  |  |  | |  |
| Format | Peptide pools |
Individual |
Individual peptide spots immobilized on cellulose membrane (array) | Individual peptides in 96-well plates |
Individual |
Individual |
| Applications | Broad antigen target discovery, T-cell epitope discovery, Biological screening, Neo-epitope based immune monitoring | Biomarker discovery, Humoral immune monitoring, (Multiplexed) antibody epitope mapping, detection of protein interactions | Biomarker discovery, antibody epitope mapping, detection of protein interactions | T-cell epitope discovery, antigen target identification, pathogen-spanning T-cell epitope discovery, (neo-epitope prioritization) | ELISA assays to map protein interactions | Biomedical screening assays, mapping of protein interactions, identification of enzyme substrates with standard screening systems |
| Assays | ELISpot | Binding assays with fluorescence read-out | Binding assay with colorimetric or chemi-luminescence read-out |
ELISpot | ELISA |
Binding assays with streptavidin coated beads, membranes, glass slides or microtiter plates (ELISA) |
| Peptide QC | 5% (or 100%) analyzed by LC-MS | 5% analyzed by LC-MS + control incubation of final microarray slide | 5% analyzed by MALDI-TOF-MS |
5% (or 100%) analyzed by LC-MS |
5% or 100% analyzed by LC-MS | 5% analyzed by LC-MS |
| Amount per peptide | 5-20 µg | 30 fmol | 1-5 nmol | 10 - 50 µg | flexible | 10 - 50 µg |
| Max. number of peptides | 300 peptides / pool | HD: 6912 peptides /array Multiwell: 192 peptides /array | 400 / array (20 x 20 peptides) |
96 peptides /plate | 96 peptides /plate | 96 pepides/plate |