Custom PepStar™ Peptide Microarrays

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JPT's unique PepStar™ peptide microarrays are comprised of immobilized peptides on glass slides.

Peptide microarrays (or peptide chips) are used for biomarker discovery, immunomonitoring, antibody epitope mapping, multiplexed epitope mapping or for detection and validation of protein interactions. In addition, PepStar™ peptide microarray technology also enables enzyme profiling.

Chemical peptide synthesis is followed by peptide immobilization on glass slides via a flexible linker. Using chemoselective coupling, we generate peptide microarrays displaying directed and covalently attached peptides. Multiple identical copies of each peptide microarray can be prepared with flexible layouts at unmatched prices and turnaround times.

PepStar peptide microarrays are available in the classical High-Content PepStar microarray format, displaying up to 6912 peptides in triplicate or in a convenient Multiwell PepStar Format. Multiwell peptide microarrays contain 21 subarrays with up to 192 peptides each, that can be incubated separatedly but in parallel and with very small sample volume. Contact us for further information!

Have a look at our incubation and assay services for epitope mapping and discovery:

Antibody Epitope Discovery Service and Antibody Response Profiling.

Applications

Applications for Custom PepStar™ Peptide Microarrays

Mapping of immunodominant regions in antigens
Antibody epitope mapping and optimization
Elucidation of signal transduction pathways
Identification of substrates for orphan enzymes
Optimization of known enzyme substrates
Reliable detection of contaminating enzyme activities

Application Notes:

The Challenge of Antigen Sequence Diversity: Solutions with ULTRA‐Peptide Libraries
Reimer (2015) Full Text
Multiple Sclerosis and Epstein‐Barr Virus Infection. An Epitope Mapping Study
Reimer et al. (2014) Full Text
Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic antibody IMAB362
Daneschar et al. (2014) Full Text
Antibody Signatures Defined by High-Content Peptide Microarray Analyses
Schutkowski et al. (2009) Full Text
A Modular Approach for Epitope Discovery and High-Resolution Profiling of Humoral Immune Responses
Pawlowski et al. (2013) Full Text

Benefits

Benefits of Custom PepStar™ Peptide Microarrays

Hundreds of identical microarray copies from one peptide synthesis
Peptides that are free of truncated sequences
Only small amounts of your precious samples needed for incubation
Low background on glass surface & read-out via fluorescence
Peptide ELISAs and individual custom peptides for validation
Flexible peptide microarray layout, e.g. for automated incubation and multiwell microarray incubation
Assay services available

Testimonials

Testimonials for Custom PepStar™ Peptide Microarrays

"The RV 144 HIV trial is considered as one of first successful HIV vaccine trials. It has become clear that the V2 loop of gp120 is an important site for immunogenicity and protection from HIV infection. The use of JPT's PepStar™ microarray technology has been very useful for the correlation of the clincial outcome with humoral immune responses. As have the cyclic peptides been from JPT to validate these findings!"
Jeff Currier, PhD, Walter Reed Army Institute, Rockville, Maryland, USA

"Over the past five years my research team has been active in the development of species-specific peptide arrays. Our ability to advance this technology into new research areas has been dependent on working with the highest quality of arrays. In this regard, JPT’s commitment to excellence has been a critical foundation for the success of our research program. I have been consistently impressed with their customer service, technical expertise and professionalism. When it comes to peptide synthesis there are no other options for our group…JPT is the best."
Scott Napper, PhD, Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Canada

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References

Quality Assurance

All production is ISO 9001:2015 certified.