Take Control of Your T Cell Assays
Robust and reproducible T cell assays depend on the use of well-defined positive controls and negative controls to ensure assay performance, data comparability, and correct interpretation of results.
JPT offers a comprehensive portfolio of peptide-based stimulation controls designed to support functional T cell assays such as ELISpot, intracellular cytokine staining (ICS), and flow cytometry–based functional assays.
Peptide-Based Positive Controls for Immune Monitoring
Our control peptide pools consist of well-characterized T cell epitopes derived from common infectious pathogens with broad population coverage across different HLA backgrounds and biogeographical regions. These peptide pools provide a physiologically relevant positive control to confirm antigen-specific T cell responsiveness in human samples.
Peptide-based controls are particularly well suited for applications where antigen processing and HLA restriction are required, making them ideal for peptide-driven assays and immune monitoring studies.
Benefits of Peptide Controls over PHA, ConA, Ionomycin
In addition to peptide pools, there a widely used functional T cell stimulants as assay controls, including: PHA (Phytohemagglutinin), ConA (Concanavalin A).
These reagents serve as non-specific positive controls to verify cell viability, activation potential, and assay functionality independent of antigen specificity.
However, if you are looking for specific positive controls that check for antigen-specific stimulation, you should consider peptide control pools!
Choosing the Right Positive Control for Your Assay
Different assays and experimental goals require different types of controls. We offer a list of peptide pools designed as efficient and reliable T cell assay controls.
To support assay selection, a comparison table below summarizes all available positive controls, highlighting their antigen specificity.
| Control Pool | Quality Grades | Organisms |
| CEF (standard) & CEF (extended) | >70% >90% | CMV, EBV, Influenza A |
| CEFT Pool | >70% >90% Premium Grade* | CMV, EBV, Influenza A, C. tetani |
| CEFT (MHC I) & CEFT (MHC II) | >70% >90% | CMV, EBV, Influenza A, C. tetani |
| CEFX Pool | >70% >90% Premium Grade* | CMV, EBV, Influenza A, C. tetani, Coxsackievirus B4, H. influenza, H. pylori, HAdV 5, HHV 1, HHV 2, HHV 3, HHV 6, HPV, JC polyomavirus, Measles virus, RuV, Toxoplasma gondii, VACV |
| CEFSX Pool | >70% >90% Premium Grade* | CMV, EBV, Influenza A, C. tetani, Coxsackievirus B4, H. influenza, H. pylori, HAdV 5, HHV 1, HHV 2, HHV 3, HHV 6, HPV, JC polyomavirus, Measles virus, RuV, Toxoplasma gondii, VACV, SARS-CoV-2 |
| CEFX (MHC I) & CEFX (MHC II) | >70% >90% | CMV, EBV, Influenza A, C. tetani, Coxsackievirus B4, H. influenza, H. pylori, HAdV 5, HHV 1, HHV 2, HHV 3, HHV 6, HPV, JC polyomavirus, Measles virus, RuV, Toxoplasma gondii, VACV |
| Cyno CMVX | >70% | Cynomolgus macaque cytomegalovirus (CyCMV) |
| EF Pool | >70% | EBV, Influenza A |
| EFX Pool | >70% >90% | EBV, C. tetani, Coxsackievirus B4, H. influenza, H. pylori, HAdV 5, HHV 1, HHV 2, HHV 3, HHV 6, HPV, JC polyomavirus, Measles virus, RuV, Toxoplasma gondii, VACV |
| PepMix CMV (pp65) | >70% >90% Premium Grade* | CMV (overlapping peptide scan of pp65) |
* Premium Grade includes recurrent performance and stability testing with updated CoA after each test.
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Proven Tools with Extensive Scientific Validation
JPT control peptide pools are widely used in academic, clinical, and industrial immunology research. Numerous peer-reviewedpublications demonstrate their performance in immune monitoring studies, vaccine development, and immunotherapy research.
You can explore selected publications using our control pools or learn more about PepMix™ peptide pools for immune monitoring.
Expert Support for Assay Design
If you have questions about selecting the appropriate positive control, optimizing your ELISpot assay, or designing your peptide-based T cell stimulant, our experienced customer support team is happy to assist you.
Please do not hesitate to contact us for further information on our peptide tools.