The World of PepMix™
PepMix™ Peptide Pools mimic a protein by representing all T cell stimulating overlapping peptides. They stimulate antigen-specific T cell responses and can be used for a wide variety of applications ranging from antigen and epitope discovery, immune monitoring in research and clinical trials to the development of diagnostic tests and adoptive cell therapy.
JPT's PepMix™ became the gold standard for antigen-specific T cell stimulation in assays such as ELISPOT and flow cytometry because of its
- Highest quality warranted by ISO 9001 certified manufacturing
- Complete protein coverage guaranteed for all protein-spanning peptide pools
- High batch-to-batch consistency
- Proprietary capping process to avoid de novo epitopes
- Minimized cross contamination with unrelated peptides
- State-of-the-art elimination of residual toxic chemicals
- Low bioburden workflow
- Largest selection of catalog peptide pools in the market; continuously updated to cover recent developments (e.g. outbreaks and viral variants)
PepMix™ Applications
PepMix™ Peptide Pools are used for a variety of applications:
- Antigen-specific stimulation in T cell assays (e.g. in ELISpot, cytokine staining, cell-mediated cytotoxicity or proliferation assays)
- In-vitro T cell expansion and dendritic cell pulsing
- Immune monitoring in research and in clinical trials
- Monitoring tool for virus outbreaks
- Cellular immune response profiling
- T cell epitope identification
- Assay control and standardization of T cell assays
Have a look at scientific publications using PepMix™!
PepMix™ Formats
PepMix™ & PepMix™ Collections | SpotMix (PLUS) | EMPS | PepSup | Ultra Pools | Control Pools | PepMix™ Pan Select | |
| Link to product page |  |  |  |  |  | |  |
| Peptide pool design | protein-spanning overlapping peptide pools |
protein-spanning overlapping peptide pools | matrix pools & individual peptides | any peptide pool diluted in PepSup medium | specialist peptide pool reflecting sequence variety | selected MHC-restricted epitopes in a peptide pool | immune dominant epitopes of an organism in one peptide pool |
| Application example | antigen-specific stimulation in cellular assays | antigen discovery | epitope mapping | ready-to-use whole blood stimulation | stimulation of T cells regardless of viral variant | control of functional T cell assays | strong stimulation of T cells |
| Purity | research plus grade (each peptide is main product) development grade or trial grade (each peptide is purifed to 70% / 90%) clinical grade (high purity with addtional QC and documentation) | research grade (each peptide is detectable) | development grade (each peptide is purified to >70%) |
depending on source pool |
development grade (each peptide is purified to >70%) |
development grade or trial grade (each peptide is purified to 70% / 90%) |
research plus grade (each peptide is main product) development grade (each peptide is purified to >70%) |
| Amount | 15 nmol per peptide; flexible for custom PepMix | 5 - 20 µg per peptide | 5 µg per peptide in each aliquot |
15 nmol per peptide; flexible for custom PepMix |
15 nmol per peptide |
15 nmol per peptide |
15 nmol per peptide |
| Delivery format | freeze-dried in vial |
freeze-dried in vial |
freeze-dried in 96 tube rack |
diluted in PepSup medium |
freeze-dried in vial |
freeze-dried in vial |
freeze-dried in vial |
| Peptide QC (HPLC-MS) | all peptides | 5% of peptides |
all peptides |
all peptides |
all peptides |
all peptides |
all peptides |
| Pool QC | presence of each peptide in pool ensured | N/A |
presence of each peptide in pool ensured |
presence of each peptide in pool ensured |
presence of each peptide in pool ensured |
presence of each peptide in pool ensured |
presence of each peptide in pool ensured |
| Custom or catalog? | both | custom | catalog |
custom |
catalog |
catalog |
catalog |
Please contact us in case of questions!
You might also be interested in