B-Cell Epitope Mapping

JPT offers a large variety of platforms for B-cell epitope discovery, antibody epitope mapping, humoral immune response profiling, elucidation of antibody binding pattern, and validation of protein interactions.

  • PepStar™ peptide microarray technology for highly reproducible discovery and monitoring studies using patient sera
  • PepSpots - membrane bound peptide arrays for fast and low cost epitope mapping
  • Peptide ELISA
  • High-quality assay service  

PepStar™ Peptide Microarrays

JPT offers PepStar™ peptide microarrays for profiling of cancer specific samples or antibodies, detection of epitopes or neo-epitopes, vaccine target identification, and monitoring epitope spreading. Peptide microarrays are reliable and efficient tools for screening assays such as the characterization of potential therapeutic antibodies, the identification of novel biomarkers, and the elucidation of protein/protein interactions.
Peptide microarrays can display overlapping peptide scans through antigens, or any other peptide sequences. You define content and layout and we provide economic and fast production of customized PepStar™ peptide micorarrays in our regulated clean-room environment. Or you can choose one of our off-the-shelf peptide microarrays that display overlapping peptide scans through hundreds of antigenic proteins.

PepSpots Peptide Arrays on Membrane

PepSpots are membrane bound peptide arrays used for efficient and inexpensive antibody epitope mapping and protein-protein interaction studies. Peptides are synthesized by our proprietary SPOT technology directly on hydrophilic cellulose membranes, which minimize non-specific binding. Easy detection using standard ELISA protocols.

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Antibody Response Profiling

This modular assay service for antibody response profiling and biomarker discovery in patient samples analyzes humoral immune response in three steps. The three assay service modules can be combined or utilized individually according to your requirements.
  • High throughput antibody response profiling or biomarker screening, using thousands of custom peptides
  • Verification of the peptide hits for hundreds of samples
  • Validation of results by robust custom peptide ELISA

Peptide ELISA

We offer customized peptide-coated ELISA plates (enzyme-linked immunosorbent assay). ELISA is a well-established analytical and highly sensitive immunological assay for proteins but requires detailed experimental know-how for using peptides. Peptide ELISA is used for mapping of epitopes and definition of protein interaction sites or as validation assay to confirm results obtained with JPT’s peptide microarrays.

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use of mapping test

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