Mass Spectrometry Based Proteomics

Proteomics is the study of a cell's protein inventory at different times by protein identification and quantification. The application of LC-MS/MS has tremendously facilitated this process. Generally, samples containing relevant proteins are digested into peptides and analyzed by LC-MS/MS as a surrogate measurement of protein levels. To increase accuracy of detection and to enable protein quantitation, chemically synthesized stable isotope-labeled (SIL) peptides are added to samples. As traditional assembly and quantitation methods for such peptides are tedious and expensive, JPT has developed unique and highly efficient procedures for both fast and low-cost synthesis as well as absolute quantitation of SIL peptides.

SpikeTides™ & SpikeMix™ Reference Peptides for Targeted Proteomics

Our proprietary peptide synthesis technology enables ultra fast and inexpensive provision of light or stable-isotope labeled reference peptides and peptide pools for proteome-wide profiling by mass spectrometry. Our SpikeTides™ reference peptides and SpikeMix™ peptide pools enable robust, multiplexed identification and relative quantification of protein expression levels.

  • Inexpensive & fast delivery (10 000 peptides/ week)
  • For targeted proteomics (protein detection and protein quantification by mass spectrometry)
  • Monitoring of cellular regulation by incorporation of post-translational modifications

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Proteomics Standards

Comprehensive control and standardization kits for MS-based proteomics assays ensure the quality of your assays and help to achieve successful experiments.

  • Normalize HPLC-MS retention time
  • Compare collision energy settings
  • Monitor efficacy of carbamidomethylation
  • Check efficacy of trypsination
  • Standardize MRM assays across species

Absolute Quantified Peptides SpikeTides™ TQL

Our proprietary peptide quantitation method overcomes the limitations of traditional methods such as limited accuracy and high costs. We use our QTag, a small chemical tag attached to each peptide, allowing robust and reproducible quantitation via HPLCUV or HPLC-MS and UV. The QTag is readily released by digestion with trypsin and does not interfere with subsequent measurements. The method is very accurate and has been validated in numerous peer-reviewed papers. Please also see our paper describing the peptide quantification method in detail (PMID: PMID: 32267065)

Protein Interaction Screen on Peptide Matrix (PRISMA)

Protein-protein interactions are a hallmark of signal transmission and key to understanding regulatory mechanisms. The PRISMA assay enables you to systematically explore the interactome.

  • Identification of protein-protein interaction partners
  • Decoding the influence of post-translational modifications
  • Comparison of cell states
  • Mapping of pathways

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NeoSpikeMix for Immunoproteomics

NeoSpikeMix are customized peptide libraries forimmunopeptidomics, e.g. patient-specific HLA reference libraries or comprehensive iterations to possible epitopes around mutations. They provide fast and cheap access to reference peptides for this clinically relevant and rapidly developing field.

  • Hundreds or thousands of peptides within one week
  • Low price
  • Provides high sensitivity as needed for low abundance epitopes

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PTM Peptide Reference Standards

Despite recent improvements, PTM proteomics is still challenging. Typical problems are low endogenous abundance, low ionization intensity, changed fragmentation, limited stability during proteomics workflows, and/or complex fragmentation spectra interpretation, especially regarding correct PTM site localization.
Our defined and carefully selected synthetic PTM reference peptides and kits support PTM proteomics and help to overcome these challenges.

discovery and targeted proteomics

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