KLH conjugated synthetic peptides are essential tools in immunization protocols, particularly for the generation of high-titer anti-peptide antibodies. When peptides are conjugated to carrier proteins like Keyhole Limpet Hemocyanin (KLH), they become more immunogenic, meaning they are more likely to trigger a robust immune response. This is crucial for applications where peptide antigens alone may be insufficient in eliciting the desired antibody production.
Why Choose KLH for Peptide Conjugation?
KLH peptide conjugation is widely recognized for its effectiveness in presenting peptide epitopes in a form that mimics their natural state within proteins. This method enhances the immunogenicity of the peptide, leading to a stronger and more targeted antibody response. Keyhole Limpet Hemocyanin (KLH) is particularly favored due to its large size and complex structure, which provides multiple sites for peptide attachment, resulting in a highly immunogenic conjugate.
- KLH Conjugate Benefits:
- Mimics the native protein structure, ensuring accurate epitope presentation.
- Provides multiple conjugation sites, enhancing immunogenicity.
- Highly effective in generating high-titer antibodies.
Our Comprehensive Peptide Conjugation Service
At JPT, we offer a complete KLH conjugation service designed to meet your specific research needs. Leveraging our extensive experience, we provide:
- Peptide Synthesis and Purification: High-quality peptides are synthesized and purified according to stringent standards.
- Custom Conjugation Chemistry: We help you select the most suitable conjugation chemistry, whether it’s glutaraldehyde, carbodiimide (WSC), or succinimide esters (e.g., MBS, SMCC), tailored to your peptide’s specific requirements.
- Carrier Protein Selection: Beyond KLH, we offer a variety of carrier proteins such as bovine serum albumin (BSA), ovalbumin, and more. We assist in choosing the best carrier to ensure optimal antibody production.
Key Considerations for KLH Conjugated Peptide Synthesis
When opting for KLH conjugated synthetic peptides, several factors must be taken into account to maximize the effectiveness of your immunization strategy:
- Peptide Epitope Presentation: The conjugated immunogen should accurately represent the peptide's native epitope.
- Conjugation Chemistry: Selecting the right conjugation method (e.g., glutaraldehyde for N-terminus linkage or carbodiimide for C-terminus attachment) is crucial.
- Carrier Protein Flexibility: The carrier should allow for the correct orientation and flexibility of the peptide.
Technical Specifications of Our KLH Conjugation Services
Our KLH conjugated synthetic peptides are produced with meticulous attention to detail, ensuring high-quality outcomes:
- Crosslinking Chemistries: We offer various crosslinking options, including:
- Glutaraldehyde: Links carrier molecules to the peptide's N-terminus.
- Carbodiimide (WSC): Attaches carriers to the peptide's C-terminus.
- Succinimide Esters (e.g., MBS, SMCC): Binds free amino groups and Cys residues.
- Carrier Protein Options: In addition to KLH, we provide other carriers like BSA, ovalbumin, and thyroglobulin.
- Purification and Quality Control: Peptides are purified to eliminate low molecular weight contaminants, and QC/QA is performed using MALDI MS (except for KLH).
- Custom Conjugation for Small Molecules: Available upon request.
- Delivery: Peptide-conjugate solutions are delivered chilled, ensuring stability and integrity.
Contact Us for Expert Guidance
At JPT Peptide Technologies, we're here to support your research with personalized guidance on KLH conjugated synthetic peptides. Our experts can help you select the right conjugation chemistry, carrier protein, and tailor your peptide synthesis to meet your specific needs. Contact us today to discuss your project. We're ready to provide the expertise and solutions you need to achieve your research goals with precision and quality.
Quality Assurance
All production is ISO 9001:2015 certified.
