Translating the Human Proteome into Molecular and Digital Tools for Drug Discovery, Personalized Medicine and Life Science Research
The verification of the correct assignment of fragment ion mass spectra to peptide sequences is still a challenge in mass spectrometry based proteomics. The preferred way to achieve this is the use of reference spectra of synthetic peptide standards. Within the ProteomeTools project we synthesized >1 million individual tryptic and non-tryptic, non-modified, and modified peptides representing all human proteins and systematically created CID, HCD, ETD and EThcD spectra.
The libraries of peptides and spectra will greatly facilitate the validation of discovery proteomics data, and will form a basis for targeted proteomic assays, enable FDR estimation in large datasets or repositories leading to a better understanding of peptide fragmentation consequently improving identification algorithms and spectra acquisition. Based on the data, new prototypes and products will be developed that form the basis for advancing research in a broad range of biomedical areas, like biomarker discovery, target validation, and drug discovery.
The project was funded by Bundesministerium für Bildung und Forschung (BMBF).