PepMix™ Peptide Pools

Overlapping peptide pools (peptide mixes of overlapping peptide scans from virus or tumor antigens such as CMV peptide pools, HPV peptide pools or HIV peptide pools) or mixed antigenic peptide pools (e.g. CEF Pool with antigens from CMV, EBV and Influenza) are extremely efficient for antigen-specific T-cell stimulation in T cell assays, T-cell expansion, immune monitoring, cellular immune response profiling or T-cell epitope identification.
In addition to overlapping peptide pools that covers entire antigens, we offer selected antigen peptide pools such as CEF Pool, EF Pool and CEFT Pools. Peptide Pools can also be used to standardize T-cell assays, as synthetic pooled peptides are much more robust and reliable for T-cell stimulation (e.g. in ELISPOT, ICS, cytotoxity or proliferation assays) than proteins or lysates.

Control Peptide Pools for T-cell Assays

CEF Pool (extended) Positive Control Pool of 32 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
CEF Pool (standard) (120nmol) Positive Control Pool of 23 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (antigen specific T-cell stimulation)Info
CEF Pool (standard) (15nmol) Positive Control Pool of 23 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
CEFT MHCII Pool Pool of 14 peptides peptides selected from defined HLA class II-restricted T-cell epitopes for T cell assays (e.g. antigen specific T-cell stimulation)Info
CEFT Pool Positive Control Pool of 27 peptides peptides selected from defined HLA class I and II-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
EF Pool Positive Control Pool of 20 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
Negative Control Pool PepMix™ HIV-1 (Con B gag motif) Negative Control Pool of 123 peptides selected from Con B gag motifs of Human immunodeficiency virus strainsInfo
PepMix™ Human (Actin) Negative Control Pool of 120 peptides derived from a peptide scan through Actin, alpha skeletal muscle of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (HLA classI Ig-like C1 type domain) Negative Control Pool of 19 peptides derived from a peptide scan through HLA class I histocompatibility antigen, A-36 alpha chain of Homo sapiensInfo
PepMix™ Human (MOG) Negative Control Pool of 29 peptides derived from a peptide scan through Myelin-oligodendrocyte glycoprotein (MOG) of Homo sapiens (Human)Info
Positive Control Pool PepMix™ HCMVA (IE-1) Positive Control Pool of 120 peptides derived from a peptide scan through 55 kDa immediate-early protein 1 (IE-1) of Human cytomegalovirus (HHV-5)Info
Positive Control Pool PepMix™ HCMVA (IE-2) Positive Control Pool of 143 peptides derived from a peptide scan through 45 kDa immediate-early protein 2 (IE-2) of Human cytomegalovirus (HHV-5).Info
Positive Control Pool PepMix™ HCMVA (pp65) Positive Control Pool of 138 peptides derived from a peptide scan through 65 kDa phosphoprotein (pp65) of Human cytomegalovirus (HHV-5)Info

Peptide Pools for Infectious Diseases Antigens

Negative Control Pool PepMix™ HIV-1 (Con B gag motif) Negative Control Pool of 123 peptides selected from Con B gag motifs of Human immunodeficiency virus strainsInfo
PepMix AAV8 (capsid protein) Pool of 182 peptides derived from a peptide scan (15mers with 11 aa overlap) through Capsid protein (UniProt ID: Q8JQF8) of Adeno-associated virus – 8Info
PepMix™ AAV6 (Capsid protein VP1) Pool of 182 overlapping peptides from Capsid protein VP1 of Adeno-associated virus 6 for T cell assays (antigen specific T-cell stimulation)Info
PepMix™ BKV (capsid protein VP1) Pool of 88 peptides derived from a peptide scan through Major capsid protein VP1 of BK polyomavirus for T cell assays (e.g. ELISPOT)Info
PepMix™ BKV (capsid protein VP2) Pool of 85 peptides derived from a peptide scan through Minor capsid protein VP2 of BK polyomavirus for T cell assays (e.g. ELISPOT)Info
PepMix™ BKV (capsid protein VP2, isoform VP3) Pool of 56 peptides derived from a peptide scan through Minor capsid protein VP2, isoform VP3 of BK polyomavirus for T cell assaysInfo
PepMix™ BKV (large T antigen) Pool of 170 peptides derived from a peptide scan through Large T antigen of BK polyomavirus for T cell assays (antigen specific T-cell stimulation)Info
PepMix™ BKV (small T antigen) Pool of 41 peptides derived from a peptide scan through Small t antigen of BK polyomavirus for T cell assays (e.g. antigen specific T-cell stimulation)Info
PepMix™ Candida (MP65) Pool of 92 peptides derived from a peptide scan through Mannoprotein MP65 of Candida albicans (Yeast) for T cell assays (e.g. ELISPOT)Info
PepMix™ Collection EBV Collection of 13 peptide pools through 13 immunodominant antigens of Epstein-Barr virus (EBV)Info
PepMix™ Collection HCMV Collection of 19 peptide pools derived from scans through 19 immuonodominant antigens of Human cytomegalovirus (HHV-5)Info
PepMix™ CyCMV (GP-UL55) Pool of 210 peptides derived from a peptide scan through Glycoprotein UL55 of Cynomolgus macaque cytomegalovirus for T cell assays (e.g. ELISPOT)Info
PepMix™ CyCMV (TP-UL83) Pool of 133 peptides derived from a peptide scan through Tegument phosphoprotein UL83 of Cynomolgus macaque cytomegalovirus for T cell assays (e.g. ELISPOT)Info
PepMix™ EBV (BARF1) Pool of 53 overlapping peptides from Secreted protein BARF1 (p53) of Epstein-Barr virus (EBV) for T cell assays (e.g. ELISPOT)Info
PepMix™ EBV (BMLF1) Pool of 117 peptides derived from a peptide scan through mRNA export factor EB2 (BMLF1) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ EBV (BMRF1) Pool of 99 peptides derived from a peptide scan through DNA polymerase processivity factor BMRF1 of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ EBV (BRLF1) Pool of 149 peptides derived from a peptide scan through Transcription activator BRLF1 of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ EBV (BZLF1) Pool of 59 peptides derived from a peptide scan through Trans-activator protein BZLF1 of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ EBV (EBNA-LP) Pool of 124 peptides derived from a peptide scan through Epstein-Barr nuclear antigen leader protein (EBNA-LP) of Epstein-Barr virus (HHV4)Info
PepMix™ EBV (EBNA1) Pool of 158 peptides derived from a peptide scan through Epstein-Barr nuclear antigen 1 (EBNA1) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ EBV (EBNA2) Pool of 119 peptides derived from a peptide scan through Epstein-Barr nuclear antigen 2 (EBNA2) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ EBV (EBNA3a) Pool of 234 peptides derived from a peptide scan through Epstein-Barr nuclear antigen 3 (EBNA3a) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ EBV (EBNA3b) Pool of 279 peptides derived from a peptide scan through Epstein-Barr nuclear antigen 4 (EBNA3b) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ EBV (EBNA3c) Pool of 265 peptides derived from a peptide scan through Epstein-Barr nuclear antigen 6 (EBNA3c) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ EBV (GP350/GP340) Pool of 224 peptides derived from a peptide scan (15mers with 11 aa overlap) through Envelope glycoprotein GP350/GP340 of Epstein-Barr virus (HHV4)Info
PepMix™ EBV (LMP1) Pool of 94 peptides derived from a peptide scan through Latent membrane protein 1 (LMP1) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ EBV (LMP2) Pool of 122 peptides derived from a peptide scan through Latent membrane protein 2 (LMP2) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PepMix™ F. tularensis (Omp) Pool of 45 peptides derived from a peptide scan through Outer membrane protein of Francisella tularensis subsp. tularensis for T cell assays (e.g. ELISPOT)Info
PepMix™ HAdV-3 (hexon protein) Pool of 234 peptides derived from a peptide scan through Hexon protein of Human adenovirus 3 (HAdV-3) for T cell assays (e.g. ELISPOT)Info
PepMix™ HAdV-5 (penton protein) Pool of 140 peptides derived from a peptide scan through Penton protein of Human adenovirus 5 (HAdV-5) for T cell assays (e.g. ELISPOT)Info
PepMix™ HBV (Capsid protein) Pool of 44 peptides derived from a peptide scan through Capsid protein of Hepatitis B virus (HBV-A) for T cell assays (e.g. ELISPOT)Info
PepMix™ HBV (Capsid Protein) Ultra Pool of 150 peptides derived from a peptide scan through Capsid protein of HBV for T cell assays (e.g. ELISPOT)Info
PepMix™ HBV (Large envelope protein) Pool of 98 peptides derived from a peptide scan through Large envelope protein of Hepatitis B virus (HBV-A) for T cell assays (e.g. ELISPOT)Info
PepMix™ HCMVA (UL103) Pool of 60 peptides derived from a peptide scan through Protein UL103 of Human cytomegalovirus (HHV-5) for T cell assays (e.g. ELISPOT)Info
PepMix™ HCMVA (UL151) Pool of 82 peptides derived from a peptide scan through Orf UL151 of Human cytomegalovirus (HHV-5) for T cell assays (e.g. ELISPOT)Info
PepMix™ HCMVA (UL153) Pool of 67 peptides derived from a peptide scan through Orf UL153 of Human cytomegalovirus (HHV-5) for T cell assays (e.g. ELISPOT)Info
PepMix™ HCMVA (UL28) Pool of 92 peptides derived from a peptide scan through Uncharacterized protein UL28 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (UL32) Pool of 260 peptides derived from a peptide scan through Large structural phosphoprotein (UL32) of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (UL36) Pool of 117 peptides derived from a peptide scan through Uncharacterized protein UL36 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (UL40) Pool of 53 peptides derived from a peptide scan through Uncharacterized protein UL40 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (UL48)_ 2 subpools Pool of 2 x 281 peptides derived from a peptide scan through Deneddylase UL48 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (UL55) Pool of 224 peptides derived from a peptide scan through Envelope glycoprotein B (UL55) of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (UL82) Pool of 137 peptides derived from a peptide scan through 71 kDa phosphoprotein (UL82) of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (UL94) Pool of 84 peptides derived from a peptide scan through Capsid-binding protein UL94 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (UL99) Pool of 45 peptides derived from a peptide scan through Tegument protein UL99 of Human cytomegalovirus (HHV-5) for T cell assays (e.g. ELISPOT)Info
PepMix™ HCMVA (US24) Pool of 123 peptides derived from a peptide scan through Tegument protein US24 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (US29) Pool of 113 peptides derived from a peptide scan through Uncharacterized protein HHRF4 (US29) of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (US3) Pool of 44 peptides derived from a peptide scan through Unique short US3 glycoprotein of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HCMVA (US32) Pool of 43 peptides derived from a peptide scan through Uncharacterized protein HHRF7 (US32) of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PepMix™ HEV (ORF2) Pool of 163 peptides derived from a peptide scan (15mers with 11 aa overlap) through Capsid protein (UniProt ID: E9N3B9) of Hepatitis E virus (strain Kernow-C1)Info
PepMix™ HEV (ORF3) Pool of 26 peptides derived from a peptide scan (15mers with 11 aa overlap) through Uncharacterized protein ORF 3 (UniProt ID: E9N3B8) of Hepatitis E virus (strain Kernow-C1)Info
PepMix™ HHV (U90) Pool of 267 peptides derived from a peptide scan through Protein U90 of Human herpesvirus 6B for T cell assays (antigen specific T-cell stimulation)Info
PepMix™ HHV2 (E3 ICP0) Pool of 204 peptides derived from a peptide scan through E3 ubiquitin-protein ligase ICP0 of Human herpesvirus 2 for T cell assays (e.g. ELISPOT)Info
PepMix™ HHV2 (gD) Pool of 96 peptides derived from a peptide scan through Envelope glycoprotein D of Human herpesvirus 2 for T cell assays (e.g. ELISPOT)Info
PepMix™ HHV2 (R1) Pool of 283 peptides derived from a peptide scan through Ribonucleoside-diphosphate reductase large subunit of Human herpesvirus 2 for T cell assays (e.g. ELISPOT)Info
PepMix™ HHV2 (VP22) Pool of 73 peptides derived from a peptide scan (15mers with 11 aa overlap) through Tegument protein VP22 (UniProt ID: P89468) of Human herpesvirus 2 (strain HG52)Info
PepMix™ HHV6 (U54) Pool of 112 peptides derived from a peptide scan through U54 of Human herpesvirus 6B for T cell assays (antigen specific T-cell stimulation)Info
PepMix™ HIV (ENV) Ultra Pool of 150 15meric peptides derived from Envelope glycoprotein gp160 designed to cover the high sequence diversity of the HI virus for T cell assaysInfo
PepMix™ HIV (GAG) Ultra Pool of 150 15meric peptides derived from Gag polyprotein designed to cover the high sequence diversity of the HIV virus for T cell assays (e.g. ELISPOT)Info
PepMix™ HIV (NEF) Ultra Pool of 150 15meric peptides derived from Nef protein designed to cover the high sequence diversity of the HI virus for T cell assays (e.g. ELISPOT)Info
PepMix™ HIV (POL) Ultra Pool of 150 15meric peptides derived from Pol Polyprotein designed to cover the high sequence diversity of the HI virus for T cell assaysInfo
PepMix™ HPV 16 (Protein E6) Pool of 37 peptides derived from a peptide scan through Protein E6 of Human papillomavirus type 16 for T cell assays (e.g. ELISPOT)Info
PepMix™ HPV 16 (Protein E7) Pool of 22 peptides derived from a peptide scan through Protein E7 of Human papillomavirus type 16 for T cell assays (e.g. ELISPOT)Info
PepMix™ HPV 18 (Protein E6) Pool of 37 peptides derived from a peptide scan through Protein E6 of Human papillomavirus type 18 for T cell assays (e.g. ELISPOT)Info
PepMix™ HPV 18 (Protein E7) Pool of 24 peptides derived from a peptide scan through Protein E7 of Human papillomavirus type 18 for T cell assays (e.g. ELISPOT)Info
PepMix™ HPV16 (L1) Pool of 124 overlapping peptides from Late major capsid protein L1 (L1) of Human papillomavirus type 16 (HPV) for T cell assays (e.g. ELISPOT)Info
PepMix™ Human (Kir4.1) Pool of 92 peptides derived from a peptide scan (15mers with 11 aa overlap) through Kir4.1 (ATP-sensitive inward rectifier potassium channel 10, UniProt ID: P78508) of Homo sapiens.Info
PepMix™ Influenza A (HA /Brisbane (H1N1)) Pool of 139 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/Brisbane/59/2007(H1N1)) for T cell assaysInfo
PepMix™ Influenza A (HA /California (H1N1)) Pool of 139 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/California/07/2009(H1N1)) for T cell assaysInfo
PepMix™ Influenza A (HA/Indonesia (H5N1)) Pool of 140 peptides derived from a peptide scan (15mers with 11 aa overlap) through Hemagglutinin (UniProt ID: A1BK62) of Influenza A virus (Indonesia (H5N1))Info
PepMix™ Influenza A (MP1 (H3N2)) Pool of 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (strain A/Aichi/2/1968 H3N2) for T cell assaysInfo
PepMix™ Influenza A (MP1 /California (H1N1)) Pool of 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (A/California/08/2009(H1N1)) for T cell assaysInfo
PepMix™ Influenza A (MP1/AnnArbor (H2N2) Pool of 61 overlapping peptides from Matrix protein 1 (MP1) of Influenza A (H2N2) for T cell assays (e.g. ELISPOT)Info
PepMix™ Influenza A (MP2 /California (H1N1)) Pool of 22 peptides derived from a peptide scan through Matrix protein 2 of Influenza A virus (A/California/08/2009(H1N1)) for T cell assaysInfo
PepMix™ Influenza A (MP2/AnnArbor (H2N2) Pool of 22 overlapping peptides from Matrix protein 2 (MP2) of Influenza A (H2N2) for T cell assays (e.g. ELISPOT)Info
PepMix™ Influenza A (Neuraminidase/Turkey/1/2005(H5N1)) Pool of 110 peptides derived from a peptide scan through Neuraminidase of Influenza A for T cell assays (antigen specific T-cell stimulation)Info
PepMix™ Influenza A (Neuroamidase /California (H1N1) Pool of 115 peptides derived from a peptide scan through Neuraminidase of Influenza A virus (A/California/08/2009(H1N1)) for T cell assaysInfo
PepMix™ Influenza A (NP (H3N2)) Pool of 122 peptides derived from a peptide scan through Nucleocapsid protein of Influenza A virus (strain A/Kitakyushu/159/1993 H3N2) for T cell assaysInfo
PepMix™ Influenza A (NP/AnnArbor (H2N2) Pool of 122 overlapping peptides from Nucleoprotein (NP) of Influenza A (H2N2) for T cell assays (e.g. ELISPOT)Info
PepMix™ L.monocytogenes (LLO) Pool of 130 peptides derived from a peptide scan through Listeriolysin O of Listeria monocytogenes serovar 1/2a for T cell assays (e.g. ELISPOT)Info
PepMix™ RLCV (BZLF1) Pool of 59 peptides derived from a peptide scan through BZLF1 of Macacine herpesvirus 4 (Rhesus lymphocryptovirus) for T cell assays (e.g. ELISPOT)Info
PepMix™ RLCV (EBNA1) Pool of 125 peptides derived from a peptide scan through EBNA-1 of Macacine herpesvirus 4 (Rhesus lymphocryptovirus) for T cell assays (e.g. ELISPOT)Info
PepMix™ RSV (Fusion glycoprotein F0) Pool of 141 peptides derived from a peptide scan through Fusion glycoprotein F0 of Human respiratory syncytial virus for T cell assays (e.g. ELISPOT)Info
PepMix™ RSV (G glycoprotein) Pool of 72 overlapping peptides from Major surface glycoprotein G (mG) of Human respiratory syncytial virus (RSV) for T cell assaysInfo
PepMix™ RSV (nucleocapsid protein N) Pool of 95 peptides derived from a peptide scan through Nucleoprotein N of Human respiratory syncytial virus for T cell assays (e.g. ELISPOT)Info
PepMix™ VACV (Cell surface-binding protein, MVA105L) Pool of 74 peptides derived from a peptide scan through Cell surface-binding protein (MVA105L) of Vaccinia virus (strain Ankara) for T cell assaysInfo
PepMix™ VACV (Host range protein 2, MVA018L) Pool of 35 peptides derived from a peptide scan through Host range protein 2 (MVA018L) of Vaccinia virus (strain Ankara) for T cell assayInfo
PepMix™ VACV (L3L) Pool of 85 peptides derived from a peptide scan through Protein L3 of Vaccinia Virus (strain WR) for T cell assays (e.g. ELISPOT)Info
PepMix™ VACV (Major core protein P4a, MVA 121L)_2 Subpools Pool of 223 peptides (delivered in two subpools of 112 + 111 peptides) derived from a peptide scan through Major core protein P4a of Vaccinia virusInfo
PepMix™ VACV (MVA 074R) Pool of 106 peptides derived from a peptide scan through Putative 49.8k protein (MVA 074R) of Vaccinia virus (strain Ankara) for T cell assaysInfo
PepMix™ VACV (MVA093L) Pool of 79 peptides derived from a peptide scan through IMV heparin binding surface protein (MVA093L) of Vaccinia virus (strain Ankara) for T cell assaysInfo
PepMix™ VACV (MVA189R) Pool of 45 peptides derived from a peptide scan through Putative 21.7k protein (MVA189R) of Vaccinia virus (strain Ankara) for T cell assaysInfo
PepMix™ VZV (gE) Pool of 153 peptides derived from a peptide scan (15mers with 11 aa overlap) through Envelope glycoprotein E (UniProt ID: P09259) of Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)Info
PepMix™ VZV (IE62)_2 subpools Pool of 325 peptides (delivered in two subpools of 163 + 162 peptides) derived from a peptide scan through Transcriptional transactivator IE62 of Varicella-zoster virus (strain Oka vaccine)Info
PepMix™ VZV (IE63) Pool of 67 peptides derived from a peptide scan through Transcriptional transactivator IE63 of Varicella-zoster virus (strain Oka vaccine) for T cell assaysInfo
PepMix™ Yellow fever (NS4B) Pool of 60 peptides derived from a peptide scan through Yellow fever Non-structural protein 4B of Yellow fever virus for T cell assaysInfo
PepMix™ Zaire Ebola (GP/Kikwit-95) Pool of 176 peptides derived from a peptide scan through Envelope glyocprotein of Zaire Ebola virus for T cell assays (antigen specific T-cell stimulation)Info
PepMix™ Zaire Ebola (GP/Mayinga-76) Pool of 176 peptides derived from a peptide scan through Envelope glyocprotein of Zaire Ebola virus for T cell assays (antigen specific T-cell stimulation)Info
Positive Control Pool PepMix™ HCMVA (IE-1) Positive Control Pool of 120 peptides derived from a peptide scan through 55 kDa immediate-early protein 1 (IE-1) of Human cytomegalovirus (HHV-5)Info
Positive Control Pool PepMix™ HCMVA (IE-2) Positive Control Pool of 143 peptides derived from a peptide scan through 45 kDa immediate-early protein 2 (IE-2) of Human cytomegalovirus (HHV-5).Info
Positive Control Pool PepMix™ HCMVA (pp65) Positive Control Pool of 138 peptides derived from a peptide scan through 65 kDa phosphoprotein (pp65) of Human cytomegalovirus (HHV-5)Info

Peptide Pools for Tumor Associated Antigens

PepMix™ Arabidopsis (WRKY47) Pool of 120 peptides derived from a peptide scan through Probable WRKY transcription factor 47 of Arabidopsis thaliana (Mouse-ear cress)Info
PepMix™ Human (CEA) Pool of 173 peptides derived from a peptide scan through Carcinoembryonic antigen-related cell adhesion molecule 5 of Homo sapiens (Human)Info
PepMix™ Human (Claudin-6) Pool of 53 peptides derived from a peptide scan through Claudin-6 of Homo sapiens (Human) for T cell assays (antigen specific T-cell stimulation)Info
PepMix™ Human (Cyclin-B1) Pool of 106 peptides derived from a peptide scan through G2/mitotic-specific cyclin-B1 of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PepMix™ Human (ErbB2_ECD) Pool of 161 peptides derived from a peptide scan through Receptor tyrosine-protein kinase erbB-2 (extracellular domain) of Homo sapiensInfo
PepMix™ Human (ErbB2_ICD) Pool of 146 peptides derived from a peptide scan through Receptor tyrosine-protein kinase erbB-2 (intracellular domain) of Homo sapiens (Human)Info
PepMix™ Human (Histone H1.2) Pool of 51 peptides derived from a peptide scan through Histone H1.2 of Homo sapiens (Human) for T cell assays (antigen specific T-cell stimulation)Info
PepMix™ Human (Histone H4) Pool of 23 peptides derived from a peptide scan through Histone H4 of Homo sapiens (Human) for T cell assays (e.g. antigen specific T-cell stimulation)Info
PepMix™ Human (hTERT) Pool of 128 peptides derived from a peptide scan through Telomerase transcriptase of Homo sapiens for T cell assays (e.g. ELISPOT)Info
PepMix™ Human (MAGEA1) Pool of 75 peptides derived from a peptide scan through Melanoma-associated antigen 1 (MAGEA1) of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (MAGEA3) Pool of 76 peptides derived from a peptide scan through Melanoma-associated antigen 3 (MAGEA3) of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (MAGEA4) Pool of 77 peptides derived from a peptide scan through Melanoma-associated antigen 4 (MAGEA4) of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (Mammaglobin A) Pool of 21 peptides derived from a peptide scan through Mammaglobin-A of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PepMix™ Human (Melan-A/MART-1) Pool of 27 peptides derived from a peptide scan through Melanoma antigen recognized by T-cells 1 (MART-1) of Homo sapiens for T cell assaysInfo
PepMix™ Human (Melanocyte protein Pmel 17 gp100) Pool of 163 peptides derived from a peptide scan through Melanocyte protein Pmel 17 (gp100) of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (Metalloreductase STEAP1) Pool of 82 peptides derived from a peptide scan through Metalloreductase STEAP1 of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (Mucin-1) Pool of 115 peptides derived from a peptide scan through Mucin-1 of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (Myc protein) Pool of 107 peptides derived from a peptide scan through Myc proto-oncogene protein of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (NY-ESO-1) Pool of 43 peptides derived from a peptide scan through Cancer/testis antigen 1 (NY-ESO-1) of Homo sapiens for T cell assays (e.g. ELISPOT)Info
PepMix™ Human (P53) Pool of 96 peptides derived from a peptide scan through Cellular tumor antigen p53 of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PepMix™ Human (PAP) Pool of 94 overlapping peptides from Prostatic acid phosphatase (PAP) of Homo sapiens (human) for T cell assays (e.g. ELISPOT)Info
PepMix™ Human (Prame/OIP4) Pool of 125 peptides derived from a peptide scan through Melanoma antigen preferentially expressed in tumors (PRAME/OIP4) of Homo sapiens (Human)Info
PepMix™ Human (Prostate-specific antigen PSA) Pool of 63 peptides derived from a peptide scan through Prostate-specific antigen of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PepMix™ Human (PSMA) Pool of 185 peptides derived from a peptide scan (15mers with 11 aa overlap) through Glutamate carboxypeptidase 2 (Prostate-specific membrane antigen, Folate hydrolase 1, NAALADase I, UniProt ID: Q04609) of Homo sapiensInfo
PepMix™ Human (SOX-2) Pool of 77 peptides derived from a peptide scan through Transcription factor SOX-2 of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (SSX2) Pool of 45 peptides derived from a peptide scan through Protein SSX2 of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PepMix™ Human (Stromelysin-3, MMP11) Pool of 121 peptides derived from a peptide scan through Stromelysin-3 (MMP11) of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (Survivin) Pool of 33 peptides derived from a peptide scan through Baculoviral IAP repeat-containing protein 5 (Survivin) of Human for T cell assaysInfo
PepMix™ Human (TCRgamma alternate reading frame protein TARP) Pool of 12 peptides derived from a peptide scan through TCRgamma alternate reading frame protein of Homo sapiens (Human) for T cell assaysInfo
PepMix™ Human (TERT) Pool of 281 peptides derived from a peptide scan (15mers with 11 aa overlap) through Telomerase reverse transcriptase (UniProt ID: O14746) of Homo sapiens Info
PepMix™ Human (Tyrosinase) Pool of 117 peptides derived from a peptide scan through Tyrosinase of Homo sapiens (Human) for T cell assays (antigen specific T-cell stimulation)Info
PepMix™ Human (Tyrosinase-related protein 2) Pool of 127 peptides derived from a peptide scan through L-dopachrome tautomerase (Tyrosinase-related protein 2) of Homo sapiens (Human)Info
PepMix™ Human (WT1/WT33) Pool of 110 peptides derived from a peptide scan through Wilms tumor protein (WT33) of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PepMix™ Mouse (Survivin) Pool of 33 peptides derived from a peptide scan through Baculoviral IAP repeat-containing protein 5 of Mus musculus for T cell assays (e.g. ELISPOT)Info
PepMix™ Mouse (Tyrosinase) Pool of 117 peptides derived from a peptide scan through Tyrosinase of Mus musculus (Mouse) for T cell assays (antigen specific T-cell stimulation)Info

Custom Peptide Pools & Matrix Pools

Custom PepMixes™ Peptide Pools

You need a specific antigen, a set of neo epitopes or a specialized peptide pool layout?
We are happy to discuss your specifications and provide your tailored PepMix™, neo epitope pools or matrix pools. Our customer support team will assist you with peptide scans, sequence and pool design. Subsequently, we will chemically synthesize, purify and analyze the peptides in compliance with the requirements of T-cell assays. All peptide synthesis steps are optimized to avoid contaminations and failure sequences which may lead to false positive T-cell responses or inhibition of T-cell responses.
Pooling and aliquotation are performed using our validated protocols to ensure high quality purified pooled peptides in freeze dried aliquots for long stability.

Custom PepMix™ Production

Individual ELISPOT Peptides

We are happy to provide the individual peptides to validate results or for follow-up experiments.  Our custom peptide synthesis is quality controlled according to ISO 9001 standards to ensure batch-to-batch reproducibility!

Applications for PepMix™ Peptide Pools

Benefits of PepMix™ Peptide Pools

Overlapping peptide pools are preferable to whole proteins for antigen-specific T-cell stimulation because:

  • Equivalent or better stimulation of CD4 and CD8 cells
  • More reliable as control than phytohemagglutinin (PHA) and concanavalin A (ConA)
  • Improved responses in stored blood and PBM cells
  • High-batch-to-batch reproducibility
  • Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation
  • Prolonged shelf stability when stored freeze dried

    Testimonials for PepMix™ Peptide Pools

    "To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D .  Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids.  Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
    Dr Laura J Pallett, Infection and Immunity, University College London, UK.

    "The main focus of my research group at the Charité in Berlin is the development of novel immunotherapeutic approaches against cancer and infectious diseases. For reliable monitoring of tumor and virus specific T-cell responses, we have a permanent need for peptide synthesis and peptide pools that are produced in a regulated environment for application in a clinical environment. JPT has been a long term and dedicated partner in this regard, which continuously works on improving it's peptide based services."
    Prof. Dr. Carmen Scheibenbogen, Charité Berlin, Germany

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    Selected References for PepMix™ Peptide Pools


    Control Pools:

    Combined PI3K/Akt and Hsp90 Targeting Synergistically Suppresses Essential Functions of Alloreactive T Cells and Increases Tregs Berges et al., J. Leukoc. Biol. (2015) - PMID: 26265781
    K562-Derived Whole-Cell Vaccine Enhances Antitumor Responses of CAR-Redirected Virus-Specific Cytotoxic T Lymphocytes In Vivo
    Caruana et al., Clin Cancer Res. (2015) - PMID: 25691731
    Development of a Novel IGRA Assay to Test T Cell Responsiveness to HBV Antigens in Whole Blood of Chronic Hepatitis B Patients
    Dammermann et al., J Transl Med. (2015) - PMID: 25968473
    Profiling T Cell Activation Using Single-Molecule Fluorescence In Situ Hybridization and Flow Cytometry
    Bushkin et al., J. Immunol. (2015) - PMID: 25505292
    Guidelines For the Automated Evaluation of Elispot Assays
    Janetzki et al., Nature Protocols (2015) - PMID: 26110715
    Self-adjuvanted mRNA Vaccination in Advanced Prostate Cancer Patients: a First-in-Man Phase I/IIa Study
    Kübler et al., J Immunother Cancer. (2015) - PMID: 26082837

    Infectious Diseases:

    Characteristics of Immune Memory 10–15 years After Primary Hepatitis B Vaccination
    Hummel et al., Vaccine (2015) - PMID: n.a.
    A Novel Lipid Nanoparticle Adjuvant Significantly Enhances B Cell and T Cell Responses to Sub-unit Vaccine Antigens
    Swaminathan et al., Vaccine (2015) - PMID: 26555351
    An Immunoinformatic Approach for Identification of Trypanosoma cruzi HLA-A2-Restricted CD8+ T cell Epitopes
    Eickhoff et al., Hum Vaccin Immunother. (2015) - PMID: 26107442
    Innate Immune Activity Correlates with CD4 T Cell-associated HIV-1 DNA Decline During Latency-Reversing Treatment with Panobinostat
    Olesen et al., Journal of Virology (2015) - PMID: 26223643
    High-density Preculture of PBMCs Restores Defective Sensitivity of Circulating CD8 T Cells to Virus- and Tumor-derived Antigens
    Wegner et al., Blood (2015) - PMID: 26024876
    Metabolic Regulation of Hepatitis B Immunopathology by Myeloid-derived Suppressor Cells
    Pallett et al., Nature Medicine (2015) - PMID: 25962123
    Priming with a Simplified Intradermal HIV-1 DNA Vaccine Regimen followed by Boosting with Recombinant HIV-1 MVA Vaccine Is Safe and Immunogenic: A Phase Iia Randomized Clinical Trial
    Munseri et al., PloSOne (2015) - PMID: 25875843

    Tumor Associated Antigens:
    Survivin-specific CD4+ T Cells are Decreased in Patients with Survivin-positive Myeloma
    Locke et al., J Immunother Cancer (2015) - PMID: 25992291
    Presence of Circulating Her-2-reactive CD8 + T-Cells is Associated with Lower Frequencies of Myeloid-derived Suppressor Cells and Regulatory T Cells, and Better Survival in Elderly Breast Cancer Patients
    Kini Bailur et al., Breast Cancer Research (2015) - PMID: n.a.
    Peptide Libraries for the Comprehensive Coverage of the Tumor Mutanome for Immune Monitoring and Immuno Therapy
    von Hoegen et al., Journal for ImmunoTherapy of Cancer (2015)- PMID: n.a.
    An HPV-E6/E7 Immunotherapy Plus PD-1 Checkpoint Inhibition Results in Tumor Regression and Reduction in PD-L1 Expression
    Rice et al., Cancer Gene Therapy (2015) - PMID: 26337747
    A Phase I Trial Combining Decitabine/dendritic Cell Vaccine Targeting MAGE-A1, MAGE-A3 and NY-ESO-1 for Children with Relapsed or Therapy-Refractory Neuroblastoma and Sarcoma
    Krishnadas et al., Cancer Immunol Immunother. (2015) - PMID: 26105625
    STAT3 Signaling Contributes to the High Effector Activities of Interleukin-15-derived Dendritic Cells
    Okada et al., Immunol Cell Biol. (2015) - PMID: 25582338


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