PepSpots Peptides on Cellulose or membrane bound peptide arrays are efficient and inexpensive for antibody epitope mapping and protein-protein interaction studies.
Properties of PepSpots Peptide Arrays
- Membrane-bound peptides synthesized directly on cellulose membranes by SPOT synthesis technology (SPOT peptide synthesis technique)
- SPOT synthesis is a fast and economical technique for custom synthesis of hundreds of membrane peptides in parallel
- Easy experimental procedure (like ELISA)
- Inexpensive equipment needed
- Flexible array and library formats
For fast and reliable antibody epitope mapping and characterization the method of choice is PepSpots™. However, for high throughput screening with thousands of peptides and projects with unpurified samples (e.g. serum, lysate, blood) we recommend the use of PepStar™ Peptide Microarrays. Our customer support team will be glad to answer any questions.
Comparison of PepSpots Peptide Arrays & PepStar™ Peptide Microarrays
| PEPSPOTS | PEPSTAR™ | |
|---|---|---|
| Peptides attached via | C-terminus | N-terminus |
| Peptides purified | No | Yes |
| Read-out via | Chemiluminescence | Fluorescence |
| Regeneration possible | Yes | No |
| Reorder possible from same synthesis | No | Yes, up to several hundred |
| Immobilization of protein controls possible | No | Yes, up to 8 controls |
| Total incubation volume of diluted sample | Depends on number of peptides (several ml) | 200 - 300 µl |
| Applicable to patient samples | Limited | Yes |
| Each peptide is displayed | 1x | 3x |
| Array surface | Cellulose membrane | Glass slide |
| Minimum order size | 24 peptides | no minimum |
