Custom PepMix™ Peptide Pools

JPT offers overlapping peptide pools (peptide mixes covering viral or tumor antigens) and mixed antigen peptide pools (e.g. CEFX Ultra SuperStim & CEF Pool with antigens from CMV, EBV, Influenza and others).

 
PepMix™ Peptide Pools are extremely efficient for:
  • Antigen-specific T-cell stimulation in T cell assays (e.g. in ELISpot, cytokine staining, cell-mediated cytotoxicity or proliferation assays)
  • In-vitro T-cell expansion
  • Dendritic cell pulsing
  • Immune monitoring
  • Cellular immune response profiling
  • T-cell epitope identification
  • Standardization of T-cell assays PepMix™
PepMix™ Peptide Pools are the best because:
  • Produced under stringent ISO 9001 regulations
  • Each peptide quality controlled and guaranteed for identity and purity
  • CoA and HPLC-MS data available for each individual peptide batch
  • High batch-to-batch consistency
  • No false positive T-cell responses by contaminating deletion peptides
  • No toxic inhibition of T-cell responses due to stringent purification of each peptide
  • Minimization of endotoxin contamination due to low bioburden process
  • ADCF policy in place
  • HLA independent stimulation (for antigen spanning peptide pools)

Custom PepMixes™ Peptide Pools
You need a specific antigen, a set of neo epitopes or a specialized peptide pool layout?
We are happy to discuss your specifications and provide your tailored PepMix™, neo epitope pools or matrix pools. Our customer support team will assist you with peptide scans, sequence and pool design. Subsequently, we will chemically synthesize, purify and analyze the peptides in compliance with the requirements of T-cell assays. All peptide synthesis steps are optimized to avoid contaminations and failure sequences which may lead to false positive T-cell responses or inhibition of T-cell responses.
Pooling and aliquotation are performed using our validated protocols to ensure high quality purified pooled peptides in freeze dried aliquots for long stability.
Individual ELISpot Peptides
We are happy to provide the individual peptides to validate results or for follow-up experiments. Our custom peptide synthesis is quality controlled according to ISO 9001 standards to ensure batch-to-batch reproducibility!
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Applications

Applications for Custom PepMix™ Peptide Pools

  • Antigen-specific stimulation of T-cells
  • Immune monitoring during diseases or therapy
  • Assessment of vaccine efficacy
  • In-vitro T-cell expansion
  • T-cell epitope identification
  • Cellular immune response profiling
  • Evaluation of neo epitopes
  • Identification of the best antigen for individual patients (PepMix™ Collections)
Application Notes:

Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy
Lam et al. (2015) Full Text
Cytomegalovirus Protein Spanning PepMix™ Peptide Pools to Discover Changes in T cell immunity in the Aging Population
Kern (2012) Full Text
Qualification and Use of Peptide Libraries for Clinical Trial Immunomonitoring
Cox & Hayes (2013) Full Text
Benefits

Benefits of Custom PepMix™ Peptide Pools

  • Overlapping peptide pools are preferable to whole proteins for antigen-specific T-cell stimulation because:
  • Simultaneous detection of CD4+ and CD8+ responses in a single sample Equivalent or better stimulation T-cell responses compared to whole protein antigens
  • Antigen spanning peptide pools stimulate T-cells in an HLA independent manner
  • All epitopes covered, epitope spreading addressed
  • Purified peptide pools eliminate risk of toxic inhibition of T-cell responses
  • No false positive T-cell stimulation caused by deletion peptides
  • Superior control compared to phytohemagglutinin (PHA) and concanavalin A (ConA)
  • Improved responses in stored blood and PBM cells compared to whole proteins
  • High-batch-to-batch reproducibility
  • Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation
  • Prolonged shelf stability when stored freeze dried
Testimonials

Testimonials for Custom PepMix™ Peptide Pools

"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
Dr Laura J Pallett, Infection and Immunity, University College London, UK.
"The main focus of my research group at the Charité in Berlin is the development of novel immunotherapeutic approaches against cancer and infectious diseases. For reliable monitoring of tumor and virus specific T-cell responses, we have a permanent need for peptide synthesis and peptide pools that are produced in a regulated environment for application in a clinical environment. JPT has been a long term and dedicated partner in this regard, which continuously works on improving it's peptide based services."
Prof. Dr. Carmen Scheibenbogen, Charité Berlin, Germany

References
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TÜV Rheinland certified
Health - made in Germany
Quality Assurance

All production is performed according to ISO 9001:2015 standards.