PepSup™ Cell Activation Kit
PepSup™ is a highly standardized, peptide-based cell activation (cell stimulation) assay designed to produce short-term culture supernatants for analysis of cytokines or other mediators of interest. We create your PepSup™ Cell Activation Kit with your choice of PepMix™ Peptide Pools, Antigen Peptides or custom peptides using a specialized culture medium. A PepSup™ kit contains sufficient reagents for 6 cell stimulations with the pertinent positive and negative controls.
- High degree of standardization and hence reproducibility of cellular assays
- Well-suited for longitudinal monitoring
- Applicable for whole blood or other cell suspensions
- Monitor antigen-specific, stimulation-induced mediator secretion
|Amount/Peptide||Varying (approx. 5 to 20 µg / peptide depending on synthesis yield)||10 µg / peptide (each peptide quantified)||15 nmol (appr. 25µg) per peptide (each peptide quantified)||6x 2 µg /peptide (each peptide quantified)|
|Delivery Format||Freeze-dried||Freeze-dried||Freeze-dried||In PepSup™ cell medium|
|Peptide Purity||Purified by Dialysis||Purified by Dialysis||Purified by HPLC||Purified by HPLC|
|Peptide QC||5% peptides analyzed by LC-MS||100% peptides analyzed by LC-MS||100% peptides analyzed by LC-MS||100% peptides analyzed by LC-MS|
|Pool QC||none||none||Full QC for each peptide pool warranting presence of each peptide||Full QC for each peptide pool warranting presence of each peptide|
||Neo-epitope based immune monitoring, Antigen discovery||Clinical immune monitoring, preclinical & clinical research||Clinical immune monitoring, preclinical & clinical research, immunotherapy|
All production is performed according to ISO 9001:2015 standards.