PepSup™ is a highly standardized, peptide-based cell activation (cell stimulation) assay designed to produce short-term culture supernatants for analysis of cytokines or other mediators of interest. We create your PepSup™ Cell Activation Kit with your choice of PepMix™ Peptide Pools, Antigen Peptides or custom peptides using a specialized culture medium. A PepSup™ kit contains sufficient reagents for 6 cell stimulations with the pertinent positive and negative controls.
Benefits of PepSup Cell Activation Kit
- High degree of standardization and hence reproducibility of cellular assays
- Well-suited for longitudinal monitoring
- Applicable for whole blood or other cell suspensions
- Monitor antigen-specific, stimulation-induced mediator secretion
Watch our webinar: PepSup™ - Standardize Immune Response Monitoring
Compare PepSup Cell Activation Kit to other Antigen Formats
| SPOTMIX™ | SPOTMIX™ PLUS | PEPMIX™ | PEPSUP™ | |
|---|---|---|---|---|
| Amount/Peptide | Varying (approx. 5 to 20 µg / peptide depending on synthesis yield) | 10 µg / peptide (each peptide quantified) | 15 nmol (appr. 25µg) per peptide (each peptide quantified) | 6x 2 µg /peptide (each peptide quantified) |
| Delivery Format | Freeze-dried | Freeze-dried | Freeze-dried | In PepSup™ cell medium |
| Peptide Purity | Purified by Dialysis | Purified by Dialysis | Purified by HPLC | Purified by HPLC |
| Peptide QC | 5% peptides analyzed by LC-MS | 100% peptides analyzed by LC-MS | 100% peptides analyzed by LC-MS | 100% peptides analyzed by LC-MS |
| Pool QC | none | none | Full QC for each peptide pool warranting presence of each peptide | Full QC for each peptide pool warranting presence of each peptide |
| Applications | Antigen discovery |
Neo-epitope based immune monitoring, Antigen discovery | Clinical immune monitoring, preclinical & clinical research | Clinical immune monitoring, preclinical & clinical research, immunotherapy |
Quality Assurance
All production is ISO 9001:2015 certified.
