PepSpots™ Peptides on Cellulose

PepSpots™ Peptides on Cellulose or membrane bound peptide arrays are efficient and inexpensive for antibody epitope mapping and protein-protein interaction studies.
Properties of PepSpots™ Membrane Arrays:
  • Membrane-bound peptides synthesized directly on cellulose membranes by SPOT synthesis technology (SPOT peptide synthesis technique)
  • SPOT synthesis is a fast and economical technique for custom synthesis of hundreds of membrane peptides in parallel
  • Easy experimental procedure (like ELISA)
  • Inexpensive equipment needed
  • Flexible array and library formats
For fast and reliable antibody epitope mapping and characterization the method of choice is PepSpots™. However, for high throughput screening with thousands of peptides and projects with unpurified samples (e.g. serum, lysate, blood) we recommend the use of PepStar™ Peptide Microarrays. Our customer support team will be glad to answer any questions.

Comparison of PepSpots™ Peptides on Cellulose and PepStar™ Peptide Microarrays

  PEPSPOTS™ PEPSTAR™
Peptides attached via C-terminus N-terminus
Peptides purified No Yes
Read-out via Chemiluminescence Fluorescence
Regeneration possible Yes No
Reorder possible from same synthesis No Yes, up to several hundred
Immobilization of protein controls possible No Yes, up to 8 controls
Total incubation volume of diluted sample Depends on number of peptides (several ml) 200 - 300 µl
Applicable to patient samples Limited Yes
Each peptide is displayed 1x 3x
Array surface Cellulose membrane Glass slide
Applications

Applications for PepSpots™ Peptides on Cellulose

  • Antibody epitope mapping
  • Functional characterization of mapped epitopes (e.g substitutional or truncation analyses):
    Which amino acids are critical for binding?
    Which amino acids are so called "key-residues”?
  • Characterization of protein-protein contact sites, such as:
    • Receptor-ligand
    • Enzyme-substrate
    • Protein modules involved in signal transduction processes
    • Recognition sequences for heat shock proteins
Benefits

Benefits of PepSpots™ Peptides on Cellulose

  • Rapid, low-cost and accurate synthesis using robotics (SPOT Technology)
  • Hydrophilic surface of cellulose membranes
  • Minimization of unspecific hydrophobic interactions with target molecule
  • Easy detection using standard ELISA protocols
Testimonials

Testimonials for PepSpots™ Peptides on Cellulose

"Our teams at the Vrije Universiteit Brussel deal with a wide variety of projects in different areas of applied biological sciences. For many of them we are in need of peptide related tools and services ranging from peptide libraries and highly purified peptides to peptide arrays. In our search for an integrated and reliable provider we came across JPT. Meanwhile, we are collaborating with JPT for many years and are very satisfied with the relationship which led to several well received publications. Especially, their unique array technology helped us to enhance our knowledge on different protein protein interactions such as Chaperone-substrate recognition."
Prof. Joost W.H. Schymkowitz & Prof. Frederic Rousseau, VIB Switch Laboratory, Vrije Universiteit Brussels, Belgium
References

References for PepSpots™ Peptides on Cellulose

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Health - made in Germany
Quality Assurance

All production is performed according to ISO 9001:2015 standards.