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Product Description

Positive Control Pool of 14 peptides selected from defined HLA class II-restricted T-cell epitopes for T cell assays (antigen specific T-cell stimulation).

Amount: 120 nmol /peptide
Purity: Development Grade: each peptide purified to > 70% (HPLC/MS)
(equivalent to an average purity of 85%)
Delivery Format: Freeze-dried in glass vial
Application(s): T-cell Immunity
Condition(s)/Topic(s): Control, Tetanus, Infectious mononucleosis, Cytomegalovirus disease, Influenza, Flu-like disease
Standard Delivery Time: 2-5 days

PepMix™ Peptide Pools
Protein-spanning mixtures of overlapping peptides (PepMix™) are extremely efficient for
- Antigen-specific T cell stimulation in T cell assays (e.g. in ELISpot, ICS, cell-mediated cytotoxicity or proliferation assays)
- In-vitro T cell expansion
- Dendritic cell pulsing
- Immune monitoring
- Cellular immune response profiling
- T cell epitope identification
- Standardization of T cell assays

Benefits of PepMix™
- Production ISO 9001 certified
- Each peptide quality controlled and guaranteed for identity and purity
- CoA and HPLC-MS data available
- High batch-to-batch consistency
- No false positive T cell responses by contaminating deletion peptides
- No toxic inhibition of T cell responses due to stringent purification of each peptide
- Minimization of endotoxin contamination due to low bioburden process
- ADCF policy in place
- HLA independent stimulation (with antigen spanning peptide pools)


References for CEFT MHC-II Pool

Read References with
PepMix™ Peptide Pools for Infectious Diseases
COVID-19 related PepMixes™
PepMix™ Control Pools

Application Notes
Peptide Tools to Support the Fight against COVID-19
Alem et al (2020) Full text
Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy
Lam et al. (2015) Full text
Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation
Gary et al. (2015) Full text

"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our go-to company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK


Properties of CEFT MHC-II Pool

Properties Values
Amount: 15 nmol (approx. 25µg) / peptide for stimulation of 2,5 x 108 cells
Application: T-cell immunity
Category: PepMix Peptide Pools
Condition / Topic: Control, Cytomegalovirus disease, Flu-like disease, Infectious mononucleosis, Influenza, Tetanus
Layout: Freeze-dried in glass vial
Organism: Clostridium tetani, Epstein-Barr Virus (EBV), Human Cytomegalovirus (HCMV), Influenza A
Protein Name: Selected proteins
Quantification: Yes

Further Information to CEFT MHC-II Pool

Information Values
Sequence: see additional product documentation
Specifications: Defined HLA class II-restricted T-cell epitopes
TÜV Rheinland certified
Health - made in Germany
Quality Assurance

All production is ISO 9001:2015 certified.

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