Mass Spectrometry-based Discovery

SpikeTides™ are synthetic proteotypic peptides with C-terminal arginine or lysine, designed to mimic tryptic fragments of proteins. SpikeTides™ peptides can be synthesized as unlabeled and unmodified proteotypic peptides as well as heavy peptides (with stable isotope labeled amino acids), with carbamoylated (alkylated) cysteines and post-translational modifications and, last but not least, as absolutely quantified peptide standards.
Due to the flexibility of our SpikeTides™ platform, SpikeTides™ proteotypic peptides are applicable to many standard assays such as SRM assays (selected reaction monitoring) and MRM assays (multiple reaction monitoring).

Applications

Applications

  • Optimization and validation of SRM assays
  • Relative and absolute quantification of proteins
  • Selection of best proteotypic peptides showing high mass spectrometry signal response
  • Find predominant peptide fragments specific for your proteotypic peptide to be used in MRM transition
  • Develop kits to quantify entire proteomic pathways
Benefits

Benefits

  • Thousands of SpikeTides™ at unmatched turnaround times (10 000 peptides per week!)
  • SpikeTides™ absolute quantification approach is more robust and cost-effective than other peptide assays e.g. AAA
  • Fully compatible with downstream analyses like MS and HPLC
  • Chemical barcoding of complex SpikeTides™ mixtures within the JPT quantification tag possible

References

References

  • CHESS: a new Human Gene Catalog Curated from Thousands of Large-scale RNA Sequencing Experiments Reveals Extensive Transcriptional Noise
    Mihaela Pertea et al., Genome Biology (2018) - PMID: 30486838
  • Discovery and Quantification of Non-human Proteins in Human Milk
    Jing Zhu et al., J. Proteome Res. (2018) - PMID: 30489082
  • Alzheimer’s Disease Phenotype or Inflammatory Insult Does Not Alter Function of L-Type Amino Acid Transporter 1 in Mouse Blood-Brain Barrier and Primary Astrocytes
    Mikko Gynther et al., Pharmaceutical Research (2018) - PMID: 30488131
  • Multiplex Assay for Quantification of Acute Phase Proteins and Immunoglobulin A in Dried Blood Spots
    V Vidova et al., J Proteome Res. (2018) - PMID: 30408962
  • Multiple-Enzyme-Digestion Strategy Improves Accuracy and Sensitivity of Label- and Standard-Free Absolute Quantification to a Level That Is Achievable by Analysis with Stable Isotope-Labeled Standard Spiking
    JR Wiśniewski et al., Journal of Proteome Research (2018) - PMID: 30336047
  • A Rapid Non‐target Screening Method for Determining Prohibited Substances in Urine Using Liquid Chromatography/high‐resolution Tandem Mass Spectrometry
    Han et al., Drug Testing and Analysis (2018) - PMID: 30188018
  • Absolute Quantification of Targeted Meat and Allergenic Protein Additive Peptide Markers in Meat Products
    Montowska et al., Food Chemistry (2018) - PMID: n.a.
  • Quantification of Protein Markers Monitoring the Pre‐analytical Effect of Blood Storage Time Before Plasma Isolation Using 15N Metabolically Labeled Recombinant
    Ahn et al., Journal of Mass Spectrometry (2018) - PMID: n.a.
  • Dysregulation of Mucosal Membrane Transporters and Drug Metabolizing Enzymes in Ulcerative Colitis
    Erdmann et al., Journal of Pharmaceutical Sciences (2018) - PMID: 30267783
  • Improving Selectivity and Sensitivity of Protein Quantitation by LC–HR–MS/MS: Determination of Somatropin in Rat Plasma
    Bults et al., Bioanalysis (2018) - PMID: 29952231
  • Elucidation of Chemical Modifier Reactivity Towards Peptides and Proteins and the Analysis of Specific fragmentation by MALDI‐CID‐MS2
    Rühl et al., Rapid Communications in Mass Spectrometry (2018) - PMID: 29964304
  • Cerebrospinal Fluid C-Reactive Protein in Parkinson's Disease: Associations with Motor and Non-motor Symptoms
    Moghaddam et al., NeuroMolecular Medicine (2018) - PMID: 29980980
  • Extracellular Prolyl Oligopeptidase Derived from Activated Microglia is a Potential Neuroprotection Target
    Natunen et al., Basic & Clinical Pharmacology & Toxicology (2018) - PMID: 29998529
  • ProteomeTools: Systematic Characterization of 21 Post-translational Protein Modifications by LC-MS/MS Using Synthetic Peptides
    Zolg et al., Molecular and Cellular Proteomics (2018) - PMID: 29848782
  • Analysis of Histone Modifications by Mass Spectrometry
    Moritz Carl Völker‐Albert et al., Current Protocols in Protein Science (2018) - PMID: 18429056
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