Mass Spectrometry-based Discovery

SpikeTides™ are synthetic proteotypic peptides with C-terminal arginine or lysine, designed to mimic tryptic fragments of proteins. SpikeTides™ peptides can be synthesized as unlabeled and unmodified proteotypic peptides as well as heavy peptides (with stable isotope labeled amino acids), with carbamoylated (alkylated) cysteines and post-translational modifications and, last but not least, as absolutely quantified peptide standards.

Due to the flexibility of our SpikeTides™ platform, SpikeTides™ proteotypic peptides are applicable to many standard assays such as SRM assays (selected reaction monitoring) and MRM assays (multiple reaction monitoring).

Applications

Optimization and validation of SRM assays
Relative and absolute quantification of proteins
Selection of best proteotypic peptides showing high mass spectrometry signal response
Find predominant peptide fragments specific for your proteotypic peptide to be used in MRM transition
Develop kits to quantify entire proteomic pathways

Benefits

Thousands of SpikeTides™ at unmatched turnaround times (10 000 peptides per week!)
SpikeTides™ absolute quantification approach is more robust and cost-effective than other peptide assays e.g. AAA
Fully compatible with downstream analyses like MS and HPLC
Chemical barcoding of complex SpikeTides™ mixtures within the JPT quantification tag possible

References

CHESS: a new Human Gene Catalog Curated from Thousands of Large-scale RNA Sequencing Experiments Reveals Extensive Transcriptional Noise
Mihaela Pertea et al., Genome Biology (2018) - PMID: 30486838

Discovery and Quantification of Non-human Proteins in Human Milk
Jing Zhu et al., J. Proteome Res. (2018) - PMID: 30489082

Alzheimer’s Disease Phenotype or Inflammatory Insult Does Not Alter Function of L-Type Amino Acid Transporter 1 in Mouse Blood-Brain Barrier and Primary Astrocytes
Mikko Gynther et al., Pharmaceutical Research (2018) - PMID: 30488131

Multiplex Assay for Quantification of Acute Phase Proteins and Immunoglobulin A in Dried Blood Spots
V Vidova et al., J Proteome Res. (2018) - PMID: 30408962

Multiple-Enzyme-Digestion Strategy Improves Accuracy and Sensitivity of Label- and Standard-Free Absolute Quantification to a Level That Is Achievable by Analysis with Stable Isotope-Labeled Standard Spiking
JR Wiśniewski et al., Journal of Proteome Research (2018) - PMID: 30336047

A Rapid Non‐target Screening Method for Determining Prohibited Substances in Urine Using Liquid Chromatography/high‐resolution Tandem Mass Spectrometry
Han et al., Drug Testing and Analysis (2018) - PMID: 30188018

Absolute Quantification of Targeted Meat and Allergenic Protein Additive Peptide Markers in Meat Products
Montowska et al., Food Chemistry (2018) - PMID: n.a.

Quantification of Protein Markers Monitoring the Pre‐analytical Effect of Blood Storage Time Before Plasma Isolation Using 15N Metabolically Labeled Recombinant
Ahn et al., Journal of Mass Spectrometry (2018) - PMID: n.a.

Dysregulation of Mucosal Membrane Transporters and Drug Metabolizing Enzymes in Ulcerative Colitis
Erdmann et al., Journal of Pharmaceutical Sciences (2018) - PMID: 30267783

Improving Selectivity and Sensitivity of Protein Quantitation by LC–HR–MS/MS: Determination of Somatropin in Rat Plasma
Bults et al., Bioanalysis (2018) - PMID: 29952231

Elucidation of Chemical Modifier Reactivity Towards Peptides and Proteins and the Analysis of Specific fragmentation by MALDI‐CID‐MS2
Rühl et al., Rapid Communications in Mass Spectrometry (2018) - PMID: 29964304

Cerebrospinal Fluid C-Reactive Protein in Parkinson's Disease: Associations with Motor and Non-motor Symptoms
Moghaddam et al., NeuroMolecular Medicine (2018) - PMID: 29980980

Extracellular Prolyl Oligopeptidase Derived from Activated Microglia is a Potential Neuroprotection Target
Natunen et al., Basic & Clinical Pharmacology & Toxicology (2018) - PMID: 29998529

ProteomeTools: Systematic Characterization of 21 Post-translational Protein Modifications by LC-MS/MS Using Synthetic Peptides
Zolg et al., Molecular and Cellular Proteomics (2018) - PMID: 29848782

Analysis of Histone Modifications by Mass Spectrometry
Moritz Carl Völker‐Albert et al., Current Protocols in Protein Science (2018) - PMID: 18429056