Peptide Tools to Study Coronaviruses

About Coronaviruses

The coronavirus family comprises several viruses such as
  • Severe acute respiratory syndrome coronavirus (SARS-CoV)
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
  • Middle East respiratory syndrome-related coronavirus (MERS)
  • Common cold coronaviruses HCoV 229E, OC43, HKU1 and NL63
  • Various animal coronaviruses

Coronaviruses have a positive-sense single-stranded RNA genome and characteristic spikes on their surface, which create an image reminding of the solar corona. The spikes are composed of Spike proteins (S protein) which contain two subunits. Subunit S1 forms the spike head with the receptor binding domain (RBD). Subunit S2 forms the stem and enables fusion with the host cell. S1 proteins are the most variable components of the virus as they are responsible for host cell specificity. Spike protein, membrane protein (M) and envelope protein (E) are anchored in the viral envelope, a lipid bilayer.


JPT's Peptide Tools to Study Coronaviruses

JPT is an expert for manufacturing a wide variety of synthetic peptide formats for research and clinical applications in the development of immunotherapy and vaccines and immune monitoring. Our researchers constantly develop new products for well-known infectious diseases such as HIV, TB or HBV as well as newly emerging diseases such as MERS, SARS and COVID-19.

Cellular Immunity
-> PepMix™ Peptide Pools
  • Antigen-specific T-cell stimulation
  • Cellular immune monitoring
  • Vaccine target discovery
  • Blood test development
  • Cross reactivity testing between viruses
  • Cell therapy development
  • Efficient epitope mapping and identification
  • Matrix Pools and individual peptides spanning a whole antigen in one set
  • Minimal sample amount required

Humoral Immunity
-> PepStar™ Pan Coronavirus
  • Humoral immune monitoring
  • Antibody epitope discovery
  • Cross-reactivity testing (SARS-CoV-2 vs. SARS, MERS, HCoV 229E, OC43…)
  • Serological test development discovery

  • Immune monitoring
  • Antibody epitope discovery
  • Screening individual antigens of SARS-CoV-2 and SARS
  • Serological test development

  • Thousands of peptides spanning entire antigens or genomes
  • Incubation using smallest sample volumes
  • Study of antibody cross-reactivities between SARS-CoV-2 and other corona viruses
  • Verification of peptide binders with a large numbers of samples
  • Transfer of results to ELISA platform for rapid test development

  • Peptide ELISA development and service using virus antigen peptides
  • ELISA-based validation service of peptide binders identified by using JPT’s peptide microarray platform
  • Collaborative ELISA test development

Clinical Trial Immune Monitoring & Cell Therapy
  • High quality chemically synthesized antigen source for vaccine trial monitoring
  • Ancillary reagents for cellular therapy development
  • Full analytical coverage, stability testing, batch documentation and more

Proteomics
-> SpikeMix™ Stable Isotope Labeled Peptide Pools
  • Peptide pool of stable isotope labeled peptides from relevant SARS CoV-2 antigens
  • Identify and quantify virus antigens from biological samples using mass spectrometry based assays (MRM)
  • Screen 23 ready-made proteotypic peptides from SARS-CoV-2
  • Or order your custom SpikeMix™ 
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References

References


  • Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates
    Corbett et al, New England J of Med (2020)
  • Presence of SARS-CoV-2 reactive T cells in COVID-19 Patients and Healthy Donors
    Braun et al, Nature (2020)
  • Single-shot Ad26 Vaccine Protects against SARS-CoV-2 in Rhesus Macaques
    Mercado et al, Nature (2020)
  • Safety and Immunogenicity of a Modified Vaccinia Virus Ankara Vector Vaccine Candidate for Middle East Respiratory Syndrome: an Open-Label, Phase 1 Trial
    Koch et al, Lancet (2020)
  • Data, Reagents, Assays and Merits of Proteomics for SARS-CoV-2 Research and Testing
    Zecha et al, Mol Cell Proteomics (2020)
  • Self-amplifying RNA SARS-CoV-2 Lipid Nanoparticle Vaccine Candidate Induces High Neutralizing Antibody Titers in Mice
    McKay et al, Nature 2020
  • The Antiviral Potential of ERK/MAPK and PI3K/AKT/mTOR Signaling Modulation for MERS-CoV Infection as Identified by Temporal Kinome Analysis
    Kindrachuk et al, Antimicrob Agents Chemother. (2014)

Further references
Application Note
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