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CAMCheck-1000pm
Easy to use peptide kit to monitor efficiency of carbamidomethylation during proteomic sample preparation. Mixture of equimolar amounts of 3 peptides
Are you interested in your custom peptide libraries for your proteomics assays? Choose your sequence, amount and label. We will assist you along the way. Custom SpikeTides Reference Peptides.
Carbamidomethylation Check Kit (CAMCheck)
Cysteine reduction and alkylation is an integral part of all bottom-up proteomics workflows. To address the issue of reduction/alkylation reproducibility, a kit of peptides was developed. It enables the in-situ determination of disulfide reduction and cysteine alkylation conditions and reproducibility, including potential over-alkylation. The kit is compatible with routine sample digestion workflows and analysis can be performed using standard LC-MS protocols.
Benefits of JPT's CAMCheck Kit
- Compatible with bottom up proteomic workflow
- Determine disulfide reduction and cysteine alkylation efficiency and reproducibility efficiently and accurately
- Straightforward data analysis (only four peptide ion chromatograms)
- Application in native protein digests (Peptides are non-naturally occurring).
References:
Read References with Reference Peptides for Targeted Proteomics - SpikeTide™ & SpikeMix™
Application Notes
Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens
Soderblom et al., Application Note (2015) Full text
Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides
Bindel et al., Application Note (2015) Full text
Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides
Colangelo, Application Note (2014) Full text
Fast and Accurate Determination of Cysteine Reduction and Alkylation Efficacy in Proteomics Workflows
Schnatbaum, Application Note (2016) Full text
| Properties | Values |
|---|---|
| Application: | Enzymology, Proteomics |
| Category: | Proteomics Standards |
| Condition / Topic: | Control |
| Layout: | Freeze-dried in plastic vial |
| Organism: | Other/None |
| Protein Name: | Other |
| Purity: | >70% (HPLC-MS) |
| Quantification: | Yes |
| Information | Values |
|---|---|
| Sequence: | See Product Documentation |
| Specifications: | Mixture of equimolar amounts of 3 peptides |
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