RTK-1-100pmol
The peptide set (also named PROCAL - ProteomeTools Calibration Standard, Zolg et al, Proteomics 2017) enables the normalization of retention times in HPLC-MS proteomics experiments, the easy evaluation of HPLC column performance, and the calibration of collision energies.
Are you interested in your custom peptide libraries for your proteomics assays? Choose your sequence, amount and label. We will assist you along the way. Custom SpikeTides Reference Peptides.
Retention Time Standardization Kit
The Retention Time Standardization Kit is an easy-to-use kit of 40 peptides, that enables the normalization of retention times in HPLC-MS proteomics experiments, the easy evaluation of HPLC column performance, the optimization of HPLC gradients, and the calibration of collision energies. It consists of Non-naturally occuring peptides with a broad LC gradient coverage and very stable retention times over multiple injections. The use of this kit (also called PROCAL) has been published (Zolg et al, Proteomics 2017, in press).
Benefits of Retention Time Standardization Kit
- Non-naturally occuring peptides allow application in biological samples
- Broader LC gradient coverage than all competitors
- High number of peptides
- Very stable retention time in multiple injections
- Similar intensities of all peptides
- Intensive use in the ProteomeTools project
- FASTA and Skyline file available
References:
Read References with Reference Peptides for Targeted Proteomics - SpikeTide™ & SpikeMix™
Application Notes
Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens
Soderblom et al., Application Note (2015) Full text
Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides
Bindel et al., Application Note (2015) Full text
Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides
Colangelo, Application Note (2014) Full text
Fast and Accurate Determination of Cysteine Reduction and Alkylation Efficacy in Proteomics Workflows
Schnatbaum, Application Note (2016) Full text
| Properties | Values |
|---|---|
| Application: | Proteomics |
| Category: | Proteomics Standards |
| Condition / Topic: | Control |
| Layout: | Freeze-dried in plastic vial |
| Organism: | Other/None |
| Protein Name: | Other |
| Purity: | >90% (HPLC-MS) |
| Quantification: | Yes |
| Information | Values |
|---|---|
| Sequence: | See Product Documentation |
| Specifications: | Pool of 40 non-naturally occuring 11 mer peptides |
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