RTK-1-10pmol

Retention Time Standardization Kit

US$124.00

Description

Product Description

The peptide set (also named PROCAL - ProteomeTools Calibration Standard, Zolg et al, Proteomics 2017) enables the normalization of retention times in HPLC-MS proteomics experiments, the easy evaluation of HPLC column performance, and the calibration of collision energies.

Amount: 10 pmol/peptide
Purity: >90% (HPLC-MS)
Delivery Format: Freeze-dried in plastic vial
Application(s): Mass spectrometry, Proteomics
Indication(s)/Topic(s): Control
Delivery Time: 2-5 days

Retention Time Standardization Kit
The Retention Time Standardization Kit is an easy-to-use kit of 40 peptides, that enables the normalization of retention times in HPLC-MS proteomics experiments, the easy evaluation of HPLC column performance, the optimization of HPLC gradients, and the calibration of collision energies. It consists of Non-naturally occuring peptides with a broad LC gradient coverage and very stable retention times over multiple injections. The use of this kit (also called PROCAL) has been published (Zolg et al, Proteomics 2017, in press).

Benefits of Retention Time Standardization Kit
- Non-naturally occuring peptides allow application in biological samples
- Broader LC gradient coverage than all competitors
- High number of peptides
- Very stable retention time in multiple injections
- Similar intensities of all peptides
- Intensive use in the ProteomeTools project
- FASTA and Skyline file available

References

References for Retention Time Standardization Kit

References:
Read References with Reference Peptides for Targeted Proteomics - SpikeTide™ & SpikeMix™

Application Notes
Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens
Soderblom et al., Application Note (2015) Full text
Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides
Bindel et al., Application Note (2015) Full text
Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides
Colangelo, Application Note (2014) Full text

Documentation

Documentation for Retention Time Standardization Kit

Properties

Properties of Retention Time Standardization Kit

Properties Values
Application: Mass spectrometry, Proteomics
Category: Proteomics Standards
Indication / Topic: Control
Organism: Other

Further Information to Retention Time Standardization Kit

Information Values
Sequence: See Product Documentation
Specifications: Pool of 40 non-naturally occuring 11 mer peptides
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Quality Assurance

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