Absolutely quantified proteotypic peptide for direct usage as reference material in mass-spectrometry based proteomics: H-SYI-Ser(alphaDGalNAc)-SQTNDTH-K*-Qtag.
Peptide sequence(s) H-SYI-Ser(alphaDGalNAc)-SQTNDTH-K*-Qtag
Amount: 5000 pmol/peptide
Purity: purified
Delivery Format: Freeze-dried in plastic vial
Application(s): Mass spectrometry, PTM proteomics, Proteomics
Indication(s)/Topic(s): Control
Delivery Time: approx. 10 days
SpikeTides™ Quantified PTM Reference Kits & Peptides
Despite recent improvements, PTM proteomics is still challenging. Typical problems are low endogenous abundance, low ionization intensity, changed fragmentation, limited stability during proteomics workflows, and/or complex fragmentation spectra interpretation, especially regarding correct PTM site localization. Our defined and carefully selected synthetic PTM reference peptides support PTM proteomics and help to overcome these challenges and are applicable for direct usage as reference material in mass-spectrometry based proteomics.
Available PTMs as peptide kits or individual peptides: Phospho (serine, threonine, tyrosine), Arg(Me, Me2a, Me2s), Lys(Ac, Me, Me2, Me3, Cro, Suc, Mal, Biotin, Glu, For, Prop, But, Hib, GG), Sulfotyrosine, Glyco-Asn(betaDGlcNAc), Glyco-Thr(alphaDGalNAc), Glyco-Ser(alphaDGalNAc). The peptides are part of a collection of 142 peptides that comprises wild-type peptides as well as 23 different post-translational modifications (PTMs).
Quantification with Qtag: Our peptide quantitation technique shows several advantages over traditional quantitation methods. We use a small chemical Qtag attached to the peptide for precise peptide quantitation. The Qtag is released form the peptide during trypsination of the sample. Further information
Benefits of SpikeTides™ Quantified PTM Reference Kits & Peptides
- Large range of PTM reference peptides
- Selected for high recovery in LC-MS
- Stable-isotope labeled (SIL)
- Purified and absolutely quantified