Absolutely quantified proteotypic peptide for direct usage as reference material in mass-spectrometry based proteomics: H-SYI-Ser(alphaDGalNAc)-SQTNDTH-K*-Qtag.
Peptide sequence(s) H-SYI-Ser(alphaDGalNAc)-SQTNDTH-K*-Qtag
Amount: 5000 pmol/peptide
Purity: purified
Delivery Format: Freeze-dried in plastic vial
Application(s): Mass spectrometry, PTM proteomics, Proteomics
Indication(s)/Topic(s): Control
Delivery Time: approx. 10 days
SpikeTides™ Quantified PTM Reference Kits & Peptides
Despite recent improvements, PTM proteomics is still challenging. Typical problems are low endogenous abundance, low ionization intensity, changed fragmentation, limited stability during proteomics workflows, and/or complex fragmentation spectra interpretation, especially regarding correct PTM site localization. Our defined and carefully selected synthetic PTM reference peptides support PTM proteomics and help to overcome these challenges and are applicable for direct usage as reference material in mass-spectrometry based proteomics.
Available PTMs as peptide kits or individual peptides: Phospho (serine, threonine, tyrosine), Arg(Me, Me2a, Me2s), Lys(Ac, Me, Me2, Me3, Cro, Suc, Mal, Biotin, Glu, For, Prop, But, Hib, GG), Sulfotyrosine, Glyco-Asn(betaDGlcNAc), Glyco-Thr(alphaDGalNAc), Glyco-Ser(alphaDGalNAc). The peptides are part of a collection of 142 peptides that comprises wild-type peptides as well as 23 different post-translational modifications (PTMs).
Quantification with Qtag: Our peptide quantitation technique shows several advantages over traditional quantitation methods. We use a small chemical Qtag attached to the peptide for precise peptide quantitation. The Qtag is released form the peptide during trypsination of the sample. Further information
Benefits of SpikeTides™ Quantified PTM Reference Kits & Peptides
- Large range of PTM reference peptides
- Selected for high recovery in LC-MS
- Stable-isotope labeled (SIL)
- Purified and absolutely quantified
References:
Read References with Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™
Testimonials for SpikeTides™
"My unit at the Banting and Best Department of Medical Research, University of Toronto Centre for Cellular and Biomolecular Research (CCBR) works on the systematic identification and quantification of proteins and protein complexes on a global level. In addition we have started to perform targeted proteomics to study protein regulation within pathways, e.g. signaling pathways during stem cell fate decision. For this we used JPT's various SpikeTides™ peptide products to build LC-MRM assays in a fast and cost efficient manner. More recently we also found new ways of using low cost SpikeTides™_TQL as quantitative peptide standards in order to quantify protein targets more accurately. We are currently in the process of validating this technology for the accurate quantification of various predicted microRNA targets."
Prof. Andrew Emili, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
"My group studies the proteomic composition of distinct chromatin domains, the mechanisms that operate to maintain the composition of histone modifications and the associated proteins. For precise and accurate identification and quantification of histone peptides that carry multiple post-translational modifications directly from biological samples JPT's SpikeTides™ _TQL peptide standards proved to be of excellent value for our research in various projects."
Prof. Dr. Axel Imhof, Adolf-Butenandt Institute, University of Munich, Germany
| Properties | Values |
|---|---|
| Application: | Mass spectrometry, PTM proteomics, Proteomics |
| Category: | PTM Peptide Reference Standards |
| Indication / Topic: | Control |
| Layout: | Freeze-dried in plastic vial |
| Modification: | Glycosylation |
| Organism: | Other |
| Protein Name: | Selected proteins |
| amount: | 5000 pmol/peptide |
| purity: | purified |
| Information | Values |
|---|---|
| Sequence: | H-SYI-Ser(alphaDGalNAc)-SQTNDTH-K*-Qtag |
| WT Sequence: | SYISSQTNDTHK |
| Specifications: | Absolutely quantified proteotypic stable isotope-labeled peptide with Qtag |
All production is performed according to ISO 9001:2015 standards.
