ProteaseSpots are custom synthesized protease substrate peptides that are immobilized on cellulose discs. The discs are delivered ready-to use in microtiter plates. During incubation with protease samples fluorescent detection can be performed after several time points.
Applications for Protease Spots
Detection of protein cleavage sites utilizing peptide scans
Identification of novel substrates with de novo libraries
Measurement of protease activities with known substrates
Characterization of cleavage sites through substitutional analyses