Protease Spots

ProteaseSpots are custom synthesized protease substrate peptides that are immobilized on cellulose discs. The discs are delivered ready-to use in microtiter plates. During incubation with protease samples fluorescent detection can be performed after several time points.

Applications

Applications for Protease Spots

  • Detection of protein cleavage sites utilizing peptide scans
  • Identification of novel substrates with de novo libraries
  • Measurement of protease activities with known substrates
  • Characterization of cleavage sites through substitutional analyses

Benefits

Benefits of Protease Spots

  • Screen large numbers of substrates economically
  • User specified substrate sequences
  • Semi-kinetic data
  • Easy handling in 96-well plates

References

References for Protease Spots

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