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ProteaseSpots are custom synthesized protease substrate peptides that are immobilized on cellulose discs. The discs are delivered ready-to use in microtiter plates. During incubation with protease samples fluorescent detection can be performed after several time points.
Applications
Applications for Protease Spots
- Detection of protein cleavage sites utilizing peptide scans
- Identification of novel substrates with de novo libraries
- Measurement of protease activities with known substrates
- Characterization of cleavage sites through substitutional analyses
Benefits
Benefits of Protease Spots
- Screen large numbers of substrates economically
- User specified substrate sequences
- Semi-kinetic data
- Easy handling in 96-well plates
References
References for Protease Spots
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