PM-MelA

PepMix™ Human (Melan-A/MART-1)

US$545.00

In stock

Description

About PepMix™ Human (Melan-A/MART-1)

Discover our PepMix™ Human (Melan-A/MART-1), a carefully curated peptide pool designed for advanced research in T-cell immunity and cancer biomarker studies. This peptide mix comprises 27 distinct peptides derived from the Melanoma Antigen Recognized by T-cells 1 (MART-1), also known as Melan-A. MART-1 is a pivotal target in melanoma immunology, making this pool essential for researchers focused on melanoma and related cancers.

Pool of 27 peptides derived from a peptide scan through Melanoma antigen recognized by T-cells 1 (MART-1) (Swiss-Prot ID: Q16655) of Human.

PepMix™ Human (Melan-A/MART-1) - Specifications

Amount: 25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)
Purity: Development Grade: each peptide purified to > 70% (HPLC/MS)
(equivalent to an average purity of 85%)
Delivery Format: Freeze-dried in glass vial
Application(s): T-cell Immunity, Cancer biomarker research
Condition(s)/Topic(s): Cancer, Melanoma
Standard Delivery Time: 2-5 days

Detailed Features of PepMix™ Human (Melan-A/MART-1)

High-Purity Peptides for Accurate Research

Each peptide in the PepMix™ Human (Melan-A/MART-1) pool is purified to development grade with a minimum purity of 70% (HPLC/MS), averaging 85% purity. This high standard ensures that your research results are reliable and reproducible, allowing for precise stimulation and analysis of T-cell responses.

Optimal Quantity for Extensive Research

The pool contains 25 µg (approximately 15 nmol) of each peptide, providing ample material to stimulate up to 2.5 x 108 cells. This quantity is ideal for extensive experimental setups and high-throughput studies, making it suitable for various research applications.

Convenient and Reliable Delivery

The peptides are delivered freeze-dried in a glass vial, which maintains stability and extends shelf life. This format is perfect for long-term storage and ease of use in laboratory settings.

Applications of PepMix™ Human (Melan-A/MART-1)

T-Cell Immunity Studies

MART-1 peptides are instrumental in studying T-cell responses against melanoma cells. The PepMix™ pool enables researchers to explore the effectiveness of potential immunotherapies and understand the immune system's interaction with melanoma antigens.

Cancer Biomarker Research

By using this peptide pool, scientists can investigate the role of Melan-A/MART-1 as a cancer biomarker. It supports research into identifying new biomarkers for melanoma and evaluating their potential in clinical applications.

Research Topics and Conditions

Focus on Melanoma

The PepMix™ Human (Melan-A/MART-1) is specifically designed to address melanoma, a serious skin cancer. It provides valuable insights into the antigenic targets of melanoma, facilitating advancements in targeted therapies.

Customizing Your Peptide Pool

If you require a personalized peptide pool, we offer customization options. You can choose specific peptide sequences, adjust the amount, select the desired purity, and decide on the format that best suits your research needs. Our team is available to guide you through the customization process and ensure your specifications are met.

PepMix™ Peptide Pools
Protein-spanning mixtures of overlapping peptides (PepMix™) are extremely efficient for
- Antigen-specific T cell stimulation in T cell assays (e.g. in ELISpot, ICS, cell-mediated cytotoxicity or proliferation assays)
- In-vitro T cell expansion
- Dendritic cell pulsing
- Immune monitoring
- Cellular immune response profiling
- T cell epitope identification
- Standardization of T cell assays

Benefits of PepMix™
- Each peptide quality controlled and guaranteed for identity and purity
- CoA and HPLC-MS data available
- High batch-to-batch consistency
- No false positive T cell responses by contaminating deletion peptides
- No toxic inhibition of T cell responses due to stringent purification of each peptide
- Minimization of endotoxin contamination due to low bioburden process
- ADCF policy in place
- HLA independent stimulation (with antigen spanning peptide pools)

References

References for PepMix™ Human (Melan-A/MART-1)

References
Read References with
PepMix™ Peptide Pools for Tumor Associated Antigens
PepMix™ Control Pools

Application Notes
Cross-reactive T cells enhance immune responses in SARS-CoV-2 infection and vaccination
Loyal et al (2021) Full text
Peptide Tools to Support the Fight against COVID-19
Alem et al (2020) Full text
CEFX/EFX Ultra SuperStim Pools: Superior positive controls for T-cell assays with broad MHC-allele and antigen coverage
Eckey et al. (2017) Full text
Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy
Lam et al. (2015) Full text
Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation
Gary et al. (2015) Full text
PepMix™ Peptide Pools for Clinical Applications: T Cell Therapy for Viral Infections after Hematopoietic Stem Cell Transplant
Keirnan et al. (2012) Full text
Cytomegalovirus Protein Spanning PepMixTM Peptide Pools to Discover Changes in T-Cell Immunity in the Aging Population
Kern (2012) Full text

Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our go-to company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK

Documentation

Documentation for PepMix™ Human (Melan-A/MART-1)

Properties

Properties of PepMix™ Human (Melan-A/MART-1)

Properties Values
Amount: 25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)
Application: Cancer biomarker research, T-cell immunity
Category: PepMix Peptide Pools, PepMix Peptide Pools TAA
Condition / Topic: Cancer, Melanoma
Layout: Freeze-dried in glass vial
Organism: Human
Protein Name: Melanoma antigen recognized by T-cells 1 (MART-1)
Purity: >70% (HPLC-MS), Development Grade: each peptide purified to > 70% (HPLC/MS)
Quantification: Yes

Further Information to PepMix™ Human (Melan-A/MART-1)

Information Values
Sequence:
MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP
Specifications: Peptide scan (15mers with 11 aa overlap)
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