The PepMix™ Human CMV (pp65) peptide pool offers a highly effective tool for studying immune responses to Human Cytomegalovirus (HCMV). The peptides included in this pool represent T-cell epitopes that are recognized by CMV-specific CD4 and CD8 T-cells across a broad range of MHC contexts (see table below). These T-cells, when activated, typically produce pro-inflammatory cytokines such as interferon-gamma (IFN-γ), interleukin-2 (IL-2), and tumor necrosis factor (TNF).
Pool of 138 peptides derived from a peptide scan (15mers with 11 aa overlap) through 65 kDa phosphoprotein (pp65) (Swiss-Prot ID: P06725) of Human Cytomegalovirus (HCMV) - strain AD169.
PepMix™ Human CMV (pp65) - Specifications
- Amount: 25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)
- Purity: Development Grade: each peptide purified to > 70% (HPLC/MS) - (equivalent to an average purity of 85%)
- Delivery Format: Freeze-dried in glass vial
- Application(s): T-cell Immunity
- Condition(s)/Topic(s): Control, Opportunistic infections, Cytomegalovirus disease, Infection
- Standard Delivery Time: 2-5 days
Applications of PepMix™ Human CMV (pp65)
Detection and Functional Analysis
The PepMix™ Human CMV (pp65) antigen is a powerful tool for:
- Detecting T-cell responsiveness to HCMV, a common issue during HCMV infection.
- Using as a positive control in T-cell stimulation assays due to its ability to stimulate CMV-specific T-cells effectively.
Isolation and Enrichment of T-Cells
This product facilitates the isolation of CD4 and CD8 T-cells from PBMCs (Peripheral Blood Mononuclear Cells) through activation-dependent processes. By leveraging antibody-labeled magnetic beads, HCMV pp65-specific T-cells can be enriched and expanded, enabling the creation of T-cell lines or clones for in-depth studies.
Advanced Applications
JPT’s PepMix™ Human CMV (pp65) is ideal for high-quality cell assays such as:
- Cytokine production-dependent assays, including ELISPOT and flow cytometry.
- Detection of surface activation markers such as anti-CD154 or anti-CD137.
Unlike non-specific controls like staphylococcus enterotoxin B (SEB) or phytohemagglutinin (PHA), the PepMix™ pool ensures T-cell receptor-dependent activation, providing a more physiological and reliable solution for peptide-based T-cell activation assays.
Visit our webpage for more information on Peptide Tools to Study CMV.
Custom Human CMV pp65 Peptides & Pools
Are you interested in your own custom PepMix or individual HLA specific Peptides? Choose your sequence, amount, purity and pool format. We will assist you along the way.
Custom PepMix Peptide PoolsCustom Peptide SynthesisAbout PepMix™ Peptide Pools
PepMix™ peptide pools are protein-spanning mixtures of overlapping peptides designed for a variety of immunological applications, including:
- Antigen-specific T-cell stimulation in assays like ELISPOT, ICS, cell-mediated cytotoxicity, or proliferation assays.
- In-vitro T-cell expansion.
- Dendritic cell pulsing.
- Immune monitoring and profiling of cellular immune responses.
- T-cell epitope identification.
- Standardization of T-cell assays.
Benefits of PepMix™ Peptide Pools
- Guaranteed identity and purity for each peptide.
- Certificate of Analysis (CoA) and HPLC-MS data provided.
- High batch-to-batch consistency.
- No false positive T-cell responses due to contaminating deletion peptides.
- No toxic inhibition of T-cell responses thanks to stringent peptide purification.
- Minimal endotoxin contamination through low bioburden processes.
- Animal-derived component-free (ADCF) policy.
- HLA-independent stimulation using antigen-spanning peptide pools.
Additional Insights into HCMV and pp65
Role of CMV pp65 in Viral Biology
The pp65 protein, a critical tegument protein of HCMV, plays an essential role in:
- Viral replication by aiding the incorporation of viral proteins into the host cell.
- Modulating the host cell’s immune responses to ensure effective immune evasion.
- Regulating HCMV-encoded genes during viral genome expression.
Research Applications
Understanding the role of cellular proteins and HCMV genes in the context of pp65 provides insights into:
- The mechanisms of immune evasion by HCMV.
- Development of antiviral strategies targeting pp65.
- Broader implications for studying opportunistic infections in immunocompromised individuals.
