Set of 52 Matrix Pools and 336 individual overlapping peptides spanning Spike glycoprotein of MERS-CoV (Middle East respiratory syndrome-related coronavirus) for epitope identification and mapping.
Amount: 5 ug / peptide as individual peptide and in matrix pools
Purity: crude (major peak by LC-MS is guaranteed to be peptide of interest for each peptide)
Delivery Format: Freeze-dried in 5 x 96 tube racks
Application(s): Antigen specific T-cell stimulation, Immune monitoring, T-cell assays, T-cell expansion
Indication(s)/Topic(s): Covid-19, Infection, Respiratory infection
Delivery Time: 2-5 days
Epitope Mapping Peptide Set (EMPS)
Matrix Pools enable fast identification of epitopes within an antigen while consuming minimal amounts of material. From a given antigen overlapping peptides are generated and pooled into many subpools, where each peptide is present in two subpools according to a layout that we design for each antigen. In combination with all individual peptides of the matrix you are able to identify and confirm epitopes quickly and efficiently.
Benefits of Epitope Mapping Peptide Set (EMPS)
- Fast epitope identification
- Minimal amounts of sample required
- Set includes individual peptides for confirmation and validation
References:
Read References with PepMix™ Peptide Pools - Infectious Diseases & COVID-19 related publications
Application Notes
Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy
Lam et al. (2015) Full text
Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation
Gary et al. (2015) Full text
Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK
| Properties | Values |
|---|---|
| Application: | Antigen specific T-cell stimulation, Immune monitoring, T-cell assays, T-cell expansion |
| Category: | PepMix Peptide Pools |
| Indication / Topic: | Covid-19, Infection, Respiratory infection |
| Layout: | Freeze-dried in 1 x 96 tube rack, Freeze-dried in 5 x 96 tube racks |
| Organism: | MERS-CoV (Middle East respiratory syndrome-related coronavirus) |
| Protein Name: | Spike glycoprotein |
| amount: | 5 ug / peptide as individual peptide and in matrix pools |
| purity: | crude (major peak by LC-MS is guaranteed to be peptide of interest for each peptide) |
| Information | Values |
|---|---|
| Sequence: |
MIHSVFLLMFLLTPTESYVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQGRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSIRSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGVYSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRFLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVSVIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNSSLFVEDCKLPLGQSLCALPDTPSTLTPRSVRSVPGEMRLASIAFNHPIQVDQLNSSYFKLSIPTNFSFGVTQEYIQTTIQKVTVDCKQYVCNGFQKCEQLLREYGQFCSKINQALHGANLRQDDSVRNLFASVKSSQSSPIIPGFGGDFNLTLLEPVSISTGSRSARSAIEDLLFDKVTIADPGYMQGYDDCMQQGPASARDLICAQYVAGYKVLPPLMDVNMEAAYTSSLLGSIAGVGWTAGLSSFAAIPFAQSIFYRLNGVGITQQVLSENQKLIANKFNQALGAMQTGFTTTNEAFHKVQDAVNNNAQALSKLASELSNTFGAISASIGDIIQRLDVLEQDAQIDRLINGRLTTLNAFVAQQLVRSESAALSAQLAKDKVNECVKAQSKRSGFCGQGTHIVSFVVNAPNGLYFMHVGYYPSNHIEVVSAYGLCDAANPTNCIAPVNGYFIKTNNTRIVDEWSYTGSSFYAPEPITSLNTKYVAPQVTYQNISTNLPPPLLGNSTGIDFQDELDEFFKNVSTSIPNFGSLTQINTTLLDLTYEMLSLQQVVKALNESYIDLKELGNYTYYNKWPWYIWLGFIAGLVALALCVFFILCCTGCGTNCMGKLKCNRCCDRYEEYDLEPHKVHVH
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| Specifications: | Peptide scan (optimized scan into 15 mers) |
All production is performed according to ISO 9001:2015 standards.
