Ultra High-Throughput Synthesis

JPT Peptide Technologies has the unique ability to produce complex micro-scale peptides libraries made of up to 1 million single, individual peptides with rapid turnaround at unmatched pricing. Our patented SPOT technology allows for the synthesis of thousands of peptides on membrane supports. The resulting individual peptides are then transferred into microtiter plates and lyophilized.

Product Specifications:

Amounts of 50-250 nmol per peptide
Peptide length: 6-20mers
C-terminal acid or amide
Non-natural and isotopically labeled amino acids
Ready-to-use peptides in 96/384-well plates
Patented high-throughput SPOT-technology
Full analysis (LC-MS, MALDI) available

Applications

Screening projects for: bioactive peptides, T-cell epitopes, peptidic ligands, substrates, proteotypic peptides and more
Hit to lead qualification of peptidic and peptidomimetic structures
Systematic high-throughput optimization of peptidic compounds
Proteome spanning biomarker discovery
Peptide microarray production
Development of peptide pools for immune monitoring
GPCR deorphanization

Benefits

High content screening
Quick turnaround at discovery and optimization cycles
Low cost per data point

References

IgE Epitope Mapping Using Peptide Microarray Immunoassay
Gimenez et al., Methods Mol Biol. (2016) - PMID: 26490481

Molecular Diagnosis of Shrimp Allergy: Efficiency of Several Allergens to Predict Clinical Reactivity
Pascal et al., J Allergy Clin Immunol Pract. (2015) - PMID: 25769902

Protein Energy Malnutrition during Vaccination Has Limited Influence on Vaccine Efficacy but Abolishes Immunity if Administered during Mycobacterium tuberculosis Infection
Hoang et al., Infect. Immun. (2015) - PMID: 25754202

Synergistic Inhibition of PARP-1 and NF-κB Signaling Down-regulates Immune Response Against Recombinant AAV2 Vectors During Hepatic Gene Therapy
Hareendran et al., Eur J Immunol. (2015) - PMID: 26443873

The Active site of O-GlcNAc Transferase Imposes Constraints on Substrate Sequence
Pathak et al., Nat Struct Mol Biol. (2015) - PMID: 26237509

Protein Abundance Changes and Ubiquitylation Targets Identified After Inhibition of the Proteasome with Syringolin A
Svozil et al., Molecular et Cellular Proteomics (2014) - PMID: 24732913

Food Peptidomics: Large Scale Analysis of Small Bioactive Peptides - A Pilot Study
Lahrichi et al., Journal of Proteomics (2013) - PMID: 23500135