Peptide Microarray-based Profiling

JPT Peptide Technologies applies its unique PepStar™ Peptide Microarray platform to generate peptide microarrays on glass slides for biomarker discovery, development of immunediagnostics, detection and validation of protein interactions. In addition, our PepStar™ Peptide Microarray technology also enables characterization of enzyme target families such as kinases, phosphatases and proteases as well as methyl, glycosyl, acetyl transferases and lysine deacetylases.

Properties of JPT's Peptide Microarrays:
  • Peptides are immobilized on glass slides via a flexible linker
  • Chemoselective coupling generates microarrays of directed and covalently attached peptides
  • Thousands of identical copies of peptide microarrays are prepared
  • Unmatched prices and turnaround time
  • Incubation can be performed manually or automated
  • Applicable for serum, blood and other biological samples
  • Read-out is performed via fluorescence
  • Incomparable signal to noise ratio and high sensitivity

Applications

Applications

  • Mapping of immunodominant regions in antigens and entire proteomes
  • Identification and optimization of ligands and substrates
  • Humoral immune response profiling in clinical trials
  • Seromarker discovery
  • Elucidation of signal transduction pathways
  • Reliable detection of contaminating enzyme activities
Benefits

Benefits

  • Screening of tens of thousands of peptides using high content peptide microarrays
  • Screening of thousands of samples using multiwell peptide microarrays
  • Smallest sample volumes required (1µl sera, blood, purified biologics…)
  • Economical production of thousands of identical microarrays
  • High peptide purity and batch-to-batch reproducibility
  • Commercially available incubation and scanning equipment enables fast technology setup
  • Low background signal on glass surface
  • Read-out via fluorescence
  • Save time for HTS assay setup
References

References

  • Identification of B cell Epitopes Enhanced by Protein Unfolding and Aggregation
    Timothy J. Eyes et al., Molecular Immunology (2019) - PMID: 30550980
  • Application of Modified Histone Peptide Arrays in Chromatin Research
    R Mauser et al., Archives of Biochemistry and Biophysics (2018) - PMID: 30391375
  • Analysis of Binding Interfaces of the Human Scaffold Protein AXIN1 by Peptide Microarrays
    Harnoš et al., Journal of Biological Chemistry (2018) - PMID: 30166345
  • A New Tool For Studying Waterfowl Immune And Metabolic Responses: Molecular Level Analysis Using Kinome Profiling
    Pagano et al., Ecology and Evolution (2018) - PMID: n.a.
  • Analysis of Toxoplasma gondii Clonal type-specific Antibody Reactions in Experimentally Infected Turkeys and Chickens
    Maksimova et al., International Journal for Parasitology (2018) - PMID: 29969590
  • Venom allergen-like Protein 28 in Clonorchis Sinensis: Four Epitopes on its Surface and the Potential Role of Cys124 for its Conformational Stability
    Lee et al., Parasitology Research (2018) - PMID: 29876859
  • Neutralizing Antibody Responses following Long-Term Vaccination with HIV-1 Env gp140 in Guinea Pigs
    Bricault et al., Journal of Virology (2018) - PMID: 29643249
  • Coordinated Responses to Individual Tumor Antigens by IgG Antibody and CD8+ T cells Following Cancer Vaccination
    Hulett et al., Journal for ImmunoTherapy of Cancer (2018) - PMID: 29618380
  • Phosphoproteomics Analysis Identifies Novel Candidate Substrates of the Non-Receptor Tyrosine Kinase, SRMS
    Goel et al., Mol Cell Proteomics (2018) - PMID: 29496907
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