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Description
About CEFX Ultra SuperStim Pool - Premium Grade
Positive Control Pool of 176 known peptide epitopes for a broad range of HLA sub-types and different infectious agents for T-cell stimulation of populations with a diverse ethnic background.
Organisms: Clostridium tetani, Coxsackievirus B4, Haemophilus influenza, Helicobacter pylori, Human adenovirus 5, Human herpesvirus 1, Human herpesvirus 2, Human herpesvirus 3, Human herpesvirus 4, Human herpesvirus 5, Human herpesvirus 6, Human papillomavirus, Influenza A, JC polyomavirus, Measles virus, Rubella virus, Toxoplasma gondii, Vaccinia virus
CEFX Ultra SuperStim Pool - Premium Grade - Specifications
- Amount: 15 nmol (approx. 25µg) / peptide for stimulation of 2,5 x 108 cells
- Purity: Premium Grade: each peptide purified to >90% (HPLC/MS) - (equivalent to an average purity of 95%)
- Delivery Format: Freeze-dried in glass vial
- Application(s): T-cell Immunity
- Condition(s)/Topic(s): Control
- Standard Delivery Time: 2-5 days
Premium Grade Quality PepMix™
For applications where performance, reproducibility, and stability are critical, our new Premium Grade PepMixes™ feature multiple layers of ongoing quality control:
- Recurrent Performance TestingEach batch is regularly tested via ELISpot assays on two donors. Your Benefits:
- Assay standardization & validation
- Minimized performance variability
- Updated Certificate of analysis (CoA)
- Cytotox TestingEach batch is tested in an in vitro Resazurin-based assay to determine if the dissolved peptides show cytotoxic effects.
- Endotoxin TestingPh. Eur.2.6.14A LAL test or Haemotox rFC test are performed with each batch to detect endotoxins.
- Sterility TestingPh. Eur.2.6.1Each batch is incubated in tryptic soy broth. Later turbidity is determined as indicator of contaminating microorganisms.
- Plus, our trusted quality benchmarks still apply
- > 90% Purity of each peptide in the pool (via HPLC-MS)
- Validated pooling method ensuring the presence of each peptide
- Produced under stringent quality management
- CoA and HPLC-MS data available for each individual peptide batch
- Avoiding false positive T-cell responses by contaminating deletion peptides
- ADCF policy
Customize Your PepMix™ Peptide Pool!
Are you interested in your own custom PepMix™? Choose your sequence, amount, purity and pool format. We will assist you along the way. Custom PepMix™ Peptide Pools.
PepMix™ Peptide Pools
Protein-spanning mixtures of overlapping peptides (PepMix™) are extremely efficient for
- Antigen-specific T cell stimulation in T cell assays (e.g. in ELISpot, ICS, cell-mediated cytotoxicity or proliferation assays)
- In-vitro T cell expansion
- Dendritic cell pulsing
- Immune monitoring
- Cellular immune response profiling
- T cell epitope identification
- Standardization of T cell assays
Benefits of PepMix™
- Each peptide quality controlled and guaranteed for identity and purity
- CoA and HPLC-MS data available
- High batch-to-batch consistency
- No false positive T cell responses by contaminating deletion peptides
- No toxic inhibition of T cell responses due to stringent purification of each peptide
- Minimization of endotoxin contamination due to low bioburden process
- ADCF policy in place
- HLA independent stimulation (with antigen spanning peptide pools)
References
References for CEFX Ultra SuperStim Pool - Premium Grade
References
Read References with
PepMix™ Peptide Pools for Infectious Diseases
COVID-19 related PepMixes™
PepMix™ Control Pools
Application Notes
Cross-reactive T cells enhance immune responses in SARS-CoV-2 infection and vaccination
Loyal et al (2021) Full text
Peptide Tools to Support the Fight against COVID-19
Alem et al (2020) Full text
CEFX/EFX Ultra SuperStim Pools: Superior positive controls for T-cell assays with broad MHC-allele and antigen coverage
Eckey et al. (2017) Full text
Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy
Lam et al. (2015) Full text
Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation
Gary et al. (2015) Full text
PepMix™ Peptide Pools for Clinical Applications: T Cell Therapy for Viral Infections after Hematopoietic Stem Cell Transplant
Keirnan et al. (2012) Full text
Cytomegalovirus Protein Spanning PepMixTM Peptide Pools to Discover Changes in T-Cell Immunity in the Aging Population
Kern (2012) Full text
Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our go-to company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK
Documentation
Documentation for CEFX Ultra SuperStim Pool - Premium Grade
Properties
Properties of CEFX Ultra SuperStim Pool - Premium Grade
Properties | Values |
---|---|
Amount: | 15 nmol (approx. 25µg) / peptide for stimulation of 2,5 x 108 cells |
Application: | T-cell immunity |
Category: | PepMix Peptide Pools, PepMix Peptide Pools Controls |
Condition / Topic: | Control |
Layout: | Freeze-dried in glass vial |
Organism: | Other/None |
Protein Name: | Selected proteins |
Quantification: | No |
Further Information to CEFX Ultra SuperStim Pool - Premium Grade
Information | Values |
---|---|
Sequence: | see additional product documentation |
Specifications: | Defined HLA restricted T-cell epitopes |