CEFX Ultra SuperStim Pool - Premium Grade

Positive Control Pool of 176 known peptide epitopes for a broad range of HLA sub-types and different infectious agents for T-cell stimulation of populations with a diverse ethnic background.
Organisms: Clostridium tetani, Coxsackievirus B4, Haemophilus influenza, Helicobacter pylori, Human adenovirus 5, Human herpesvirus 1, Human herpesvirus 2, Human herpesvirus 3, Human herpesvirus 4, Human herpesvirus 5, Human herpesvirus 6, Human papillomavirus, Influenza A, JC polyomavirus, Measles virus, Rubella virus, Toxoplasma gondii, Vaccinia virus

CEFX Ultra SuperStim Pool - Premium Grade - Specifications

• Amount: 15 nmol (approx. 25µg) / peptide for stimulation of 2,5 x 10⁸ cells
• Purity: Premium Grade: each peptide purified to >90% (HPLC/MS) - (equivalent to an average purity of 95%)
• Delivery Format: Freeze-dried in glass vial
• Application(s): T-cell Immunity
• Condition(s)/Topic(s): Control
• Standard Delivery Time: 2-5 days

Premium Grade Quality PepMix™

For applications where performance, reproducibility, and stability are critical, our new Premium Grade PepMixes™ feature multiple layers of ongoing quality control:

• Recurrent Performance Testing
  Each batch is regularly tested via ELISpot assays on two donors.
  Your Benefits:
  • Assay standardization & validation
  • Minimized performance variability
  • Updated Certificate of analysis (CoA)
• Cytotox Testing
  Each batch is tested in an in vitro Resazurin-based assay to determine if the dissolved peptides show cytotoxic effects.
• Endotoxin Testing
  Ph. Eur.2.6.14
  A LAL test or Haemotox rFC test are performed with each batch to detect endotoxins.
• Sterility Testing
  Ph. Eur.2.6.1
  Each batch is incubated in tryptic soy broth. Later turbidity is determined as indicator of contaminating microorganisms.
• Plus, our trusted quality benchmarks still apply
  • > 90% Purity of each peptide in the pool (via HPLC-MS)
  • Validated pooling method ensuring the presence of each peptide
  • Produced under stringent quality management
  • CoA and HPLC-MS data available for each individual peptide batch
  • Avoiding false positive T-cell responses by contaminating deletion peptides
  • ADCF policy

Customize Your PepMix™ Peptide Pool!

Are you interested in your own custom PepMix™? Choose your sequence, amount, purity and pool format. We will assist you along the way. Custom PepMix™ Peptide Pools.

PepMix™ Peptide Pools
Protein-spanning mixtures of overlapping peptides (PepMix™) are extremely efficient for
- Antigen-specific T cell stimulation in T cell assays (e.g. in ELISpot, ICS, cell-mediated cytotoxicity or proliferation assays)
- In-vitro T cell expansion
- Dendritic cell pulsing
- Immune monitoring
- Cellular immune response profiling
- T cell epitope identification
- Standardization of T cell assays

Benefits of PepMix™
- Each peptide quality controlled and guaranteed for identity and purity
- CoA and HPLC-MS data available
- High batch-to-batch consistency
- No false positive T cell responses by contaminating deletion peptides
- No toxic inhibition of T cell responses due to stringent purification of each peptide
- Minimization of endotoxin contamination due to low bioburden process
- ADCF policy in place
- HLA independent stimulation (with antigen spanning peptide pools)