SP-SubSir-01

Universal Continuous Sirtuin Assay Kit

US$436.00

Description

Product Description

Assay Kit with a universal substrate peptide designed for a systematic discovery of sirtuin 1-6 modulators for a direct and continuous quantification of the deacylase activity of recombinant human sirtuins 1-6 utilizing a fluorescent signal read out.


Amount: 3 x 100 ug
Purity: >95% (HPLC-MS)
Delivery Format: Freeze-dried in 3 glass vials
Application(s): Sirtuin activity assays
Indication(s)/Topic(s): Alzheimer's disease, Cancer, Deacetylase, Sirtuin
Delivery Time: 2-5 days

Sirtuins
Silent information regulator 2 (SIR2) proteins, or sirtuins, are found in organisms ranging from bacteria to humans and represent a family of enzymes with NAD+-dependent lysine deacylase activity. The seven mammalian sirtuins, SIRT1-7, share a highly conserved NAD+-binding catalytic core domain although they exhibit distinct expression patterns, catalytic activities, and biological functions. SIRT1 and -2 have a huge variety of targets in the cytoplasm and nucleus, whereas SIRT3, -4 and -5 deacylate proteins only in mitochondria [3]. SIRT6 and -7 are deacylases localized in the nucleus.
Sirtuins catalyze the removal of acetyl and different acyl moieties from lysine side chains including propionylations, succinylations, malonylations, glutarylations, crotonylations, butyrylations and myristoylations.
By deacylating different substrate proteins, including histones, transcription factors, and metabolic enzymes, sirtuins regulate various biological processes, such as transcription, cell survival, longevity, DNA damage and repair. Sirtuins play an important role in many diseases, among them diabetes, cancer and neurodegeneration that makes these enzymes attractive targets for drug discovery.

Benefits of Universal Continuous Sirtuin Assay Kit
- Systematic discovery of sirtuin 1-6 modulators
- Direct and continuous quantification of deacylase activity
- Fluorescent signal read out

References

References for Universal Continuous Sirtuin Assay Kit

References:
Read References with Specialty Peptides

Documentation

Documentation for Universal Continuous Sirtuin Assay Kit

Properties

Properties of Universal Continuous Sirtuin Assay Kit

Properties Values
Application: Sirtuin activity assays
Category: Sirtuin Substrates
Indication / Topic: Alzheimer's disease, Cancer, Deacetylase, Sirtuin
Layout: Freeze-dried in 3 glass vials
Organism: Other/None
Protein Name: Other
amount: 3 x 100 ug, 100 ug
purity: >95% (HPLC-MS)

Further Information to Universal Continuous Sirtuin Assay Kit

Information Values
Sequence: Ac-EALPKXZGG-NH2
Specifications: TNFα-derived peptide with fluorescently labeled lysine acyl residue
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Health - made in Germany
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All production is performed according to ISO 9001:2015 standards.

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