Universal Continuous Sirtuin Assay Kit

Assay Kit with a universal substrate peptide designed for a systematic discovery of sirtuin 1-6 modulators for a direct and continuous quantification of the deacylase activity of recombinant human sirtuins 1-6 utilizing a fluorescent signal read out.

Universal Continuous Sirtuin Assay Kit - Specifications

Sirtuins
Silent information regulator 2 (SIR2) proteins, or sirtuins, are found in organisms ranging from bacteria to humans and represent a family of enzymes with NAD+-dependent lysine deacylase activity. The seven mammalian sirtuins, SIRT1-7, share a highly conserved NAD+-binding catalytic core domain although they exhibit distinct expression patterns, catalytic activities, and biological functions. SIRT1 and -2 have a huge variety of targets in the cytoplasm and nucleus, whereas SIRT3, -4 and -5 deacylate proteins only in mitochondria [3]. SIRT6 and -7 are deacylases localized in the nucleus.
Sirtuins catalyze the removal of acetyl and different acyl moieties from lysine side chains including propionylations, succinylations, malonylations, glutarylations, crotonylations, butyrylations and myristoylations.
By deacylating different substrate proteins, including histones, transcription factors, and metabolic enzymes, sirtuins regulate various biological processes, such as transcription, cell survival, longevity, DNA damage and repair. Sirtuins play an important role in many diseases, among them diabetes, cancer and neurodegeneration that makes these enzymes attractive targets for drug discovery.

Benefits of Universal Continuous Sirtuin Assay Kit
- Systematic discovery of sirtuin 1-6 modulators
- Direct and continuous quantification of deacylase activity
- Fluorescent signal read out