Protein Interaction Screen on Peptide Matrix (PRISMA)

Protein-protein interactions are a hallmark of signal transmission and key to understanding regulatory mechanisms. Therefore, we developed the Protein Interaction Screen on Peptide Matrix (PRISMA) together with our partners from Max-Delbruck-Center for Molecular Medicine (MDC) to systematically explore the interactome.
  • Peptides are synthesized on a cellulose membrane
  • Peptides of up to 25 amino acids can represent sequence diversity and contain post-translational modifications
  • The membrane is incubated with a biological sample for affinity enrichment of soluble proteins and protein complexes
  • Proteins interacting with the peptide matrix are then identified and quantified by mass spectrometry
Applications

Applications for Protein Interaction Screen on Peptide Matrix (PRISMA)

  • Identification of protein-protein interaction partners
  • Systematic exploration of the interactome
  • Influence of post-translational modifications decoded
  • Discovery of the influence of sequence diversity on protein interactions
  • Comparison of cell states
  • Identification of complexes
  • Mapping of pathways
Benefits

Benefits of Protein Interaction Screen on Peptide Matrix (PRISMA)

  • Hundreds of protein interactions can be detected and identified as potential interaction partners
  • Modulatory effects of numerous PTMs and mutations can be assessed
  • Quantification of proteins possible
  • Validated assay based on comparison with other affinity enrichment approaches, conventional immunoblotting analysis, and co-occurrence
References

References for Protein Interaction Screen on Peptide Matrix (PRISMA)

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