Improve Your Phosphoproteomic Assays
Published on 16/02/2022
We are happy to launch a new tool for standardization of mass spectrometry-based proteomic assays:
Collection of 24 phosphopeptides for standardization of phosphoproteomics workflows. Control your phospho proteomic workflow including sample workup, enrichment and LC-MS/MS. Establish HPLC retention time (RT) windows for targeted detection of phosphopeptides, perform optimization of LC-MS gradients and column performance evaluation.
Benefits:
- Non-natural occurring peptides (allowing application in biological samples)
- Non-isotopically labeled peptides (facilitating inclusion into database searches)
- Broad LC gradient coverage
- Stable RTs across multiple injections
- High Andromeda scores
- Sharp peak shapes
Have a look at our other Proteomics Standardization & Control Kits:
Normalize retention times in proteomics experiments and optimize HPLC gradients.
- Zolg el al., Proteomics 2017
- Gessulat et al., Nature Methods 2019
- Zecha et al., Molecular & Cellular Proteomics 2020
- Bian et al., Nature Communications 2020
- Wilhelm et al., Nature Communications 2021
Monitor the efficiency of carbamidomethylation during proteomic sample preparation.
- Schnatbaum et al., Application Note 2016
Estimate efficiency & reproducibility of tryptic sample preparation.
Measure challenging synthetic phosphopeptides from a set of peptides that were not originally designed as standardization kit.
- Schmidlin et al., Cell Systems 2019
Overcome the numerous challenges in PTM Proteomics (e.g. flow endogenous abundance, low ionization intensity…)
- Zolg et al., Molecular & Cellular Proteomics 2018
For more information on the use of our proteomics products, have a look at the corresponding publications and applications notes.
As always, we are looking forward to your comments and feedback!