Elevate Your Proteomics Assays: Peptide Quantification for Precision Proteomics

Published on 07/09/2023

Absolute quantified peptides are used as internal references for protein quantification in mass spectrometry-based proteomics. 

Our proprietary peptide quantitation method overcomes the limitations of traditional methods such as limited accuracy and high costs. We attach our QTag to each peptide, allowing robust and reproducible quantitation via HPLC-UV or HPLC-MS and UV. Digestion with trypsin in your sample releases the QTag and does not interfere with subsequent measurements. 

Absolute Quantified Peptides - SpikeTides TQL 

JPT’s QTag is attached to the peptide enabling peptide quantification via HPLC-UV, which is a robust, reproducible & accurate method (variation CV= 5%) with much lower cost than traditional methods. SpikeTides TQL have been used in numerous peer-reviewed publications.

Also, check our Catalog SpikeTides TQL

  • Histones
  • Metabolic Enzymes 

Clinical Proteomics Peptides - SpikeTides TQL PLUS 

Reference peptides for protein quantification in clinical proteomics are also quantified using our proprietary QTag before delivery. In addition, you yourself are able to perform an easy and reliable up-to-date peptide quantitation immediately before your experiment ensuring high accuracy for clinical applications. 

Assay Standards & Controls

  • CAMCheck Kit
    Monitor the efficiency of carbamidomethylation during proteomic sample preparation
  • Retention Time Standardization Kit (PROCAL)
    Normalize retention times in proteomics experiments and optimize HPLC gradients
  • SpikeMix™ ABRF (cross-species standard)
    Use this pool of proteotypic peptides from conserved proteins as standard across human, mouse and rat
  • TrypCheck Kit Fluorescence
    Estimate efficiency & reproducibility of tryptic sample preparation 

Did we spike your interest in our proteomics tools? Then check this further information on our website: 

Research Areas: Proteomics 
The Proteome Tools Project 
The Human Proteome Peptide Catalog
References with Proteomics Peptides 

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