Z-Nle-Lys-Arg-AMC is a fluorogenic peptide substrate developed for monitoring cathepsin B activity across acidic-to-neutral pH conditions. The Nle-Lys-Arg recognition sequence is linked to 7-amino-4-methylcoumarin (AMC); enzymatic cleavage releases AMC and generates a fluorescence-based activity signal.
Product Specifications
- Product Code: SP-CAT-093
- Sequence: Cbz-Nle-KR-AMC
- CAS Number: 1135364-14-5
- Molecular Formula: C36H50N8O7
- Molecular Weight: 706.84 g/mol
- Purity: >95% (HPLC-MS)
- Amount: 5 mg
- Format: Freeze-dried in plastic vial
- Storage: Store at -20°C
- Standard Delivery Time: 2-5 days
Applications
- Cathepsin B activity assays
- Protease measurements across acidic-to-neutral pH
- Cathepsin B selectivity studies
- Enzyme kinetics
- Cathepsin inhibitor screening
Protease Substrates for Selective Cathepsin B Studies
Z-Nle-Lys-Arg-AMC extends JPT's Protease Substrates portfolio with a fluorogenic peptide designed for cathepsin B research. Defined peptide substrates help researchers examine protease selectivity, pH-dependent activity, enzyme kinetics and inhibition under controlled assay conditions.
Benefits of Z-Nle-Lys-Arg-AMC and JPT Protease Substrates
The Nle-Lys-Arg-AMC design supports cathepsin B measurements across a broad assay pH range, making it useful when activity must be followed from acidic to near-neutral conditions. JPT Protease Substrates provide defined synthetic reagents for protease activity and inhibitor studies.
Do you need a substrate optimized for another protease or assay condition?JPT Custom Peptide Synthesis provides tailored peptide sequences, labels, modifications, purity levels and quantities to support custom enzymology and protease screening workflows.
