Alanine Scanning Library

Alanine scanning libraries are used to

  • Identify key residues in epitopes
  • Search binding parameters of enzymes
  • Study binding sites of proteins

Creating an alanine scanning library, alanine systematically substitutes the residues at each amino acid position of the original peptide i.e. identified epitope (= alanine walk). Substitution of an important or essential amino acid by alanine results in less or no binding or activity.

Use our free and easy-to-use PepSequencer  for generation of alanie scanning libraries!

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