Residual Solvent Determination

Residual solvents from peptide synthesis and purification such as DMF, Acetonitrile, DMSO or NMP can be cytotoxic or lead to false results in biological applications.

Residual solvents can be determined by gas chromatography that is able to separate chemicals in a complex sample. The peptide sample is added into a column with a gas stream (carrier gas, mobile phase) which the different chemical components pass at different rates depending on their various properties and their interaction with the column filling (stationary phase). As the chemicals exit the column, they are detected and identified.

Material requisition: 1.5 mg/sample

Time needed: 2 weeks

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