Peptide Solubility Testing

Peptide solubility is a crucial factor for in vivo bioavailability and in vitro assay robustness. Lack of full solubility at the desired effective concentration will lead to decreased activity and false negative results. Turbidimetric Solubility Assay (TSA) allows a rapid determination of solubility using small peptide amounts. Depending on applications of the target peptides, different organic (DMSO, DMF, acetonitrile etc.) and inorganic (water, aqueous buffers like PBS) solvents can be selected for solubility measurements by TSA.

  • Known amounts of lyophilized peptides are dissolved in selected solvents
  • Special conditions (e.g. ultrasound sonication; vortexing; heating treatment) can be applied to improve peptide solubility
  • If a peptide is not soluble, the solution will be turbid and produce light scattering
  • The sample is measured at 850 nm to measure any light scattering
  • A plot of turbidity vs. peptide concentration indicates the maximum concentration dissolved, which is solubility

Material requisition: 1 mg per test condition

Time needed: 2 days

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