Peptide Sequencing

The amino acid sequence represents the order of amino acids as they occur in a polypeptide chain and is critical for the biological function of a peptide. Whereas Edman degradation has been used for a long time, tandem mass spectrometry is now the method of choice for confirmation of peptide amino acid sequences. By using tandem mass spectrometry JPT is able to directly sequence peptides up to a chain length of 25 amino acids. For longer peptides we are applying peptide specific enzymatic degradation followed by subsequent sequencing of the resulting fragments.

Material requisition: 1mg

Time needed: 2 days