Peptide Quantitation / Peptide Content Determination


Due to their chemical properties lyophilized peptides may still contain traces of moisture and counter ions on protonated amino functions (N-terminus, Arg, His, Lys, etc.). This is not considered an impurity, but reduces the actual peptide content by approximately 10 to 30%.

An accurate determination of the peptide content can be performed by quantitative amino acid analysis. The analysis includes an acidic hydrolysis of the peptide, transforming the sample into free amino acids. Then amino acid analysis and quantification of total peptide content are performed.

Material requisition: 1 mg/sample

Time needed: 2 weeks

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