Peptide Purity Determination

Peptide Purity is determined by UV absorption at 220nm using RP-HPLC (reversed phase high performance liquid chromatography). This technique is used to separate, identify, and quantify components in a mixture, e.g. a target peptide and potential impurities.

  • Peptide sample in solution is pumped through a adsorbent column
  • Sample components interact differently with the adsorbent
  • Different flow rates lead to separation of components
  • UV detector generates a signal for each component at the end of the column
  • Allows for quantitative analysis of components

Material requisition: > 150 µg/sample

Time needed: 1-2 days

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