Peptide Purity Determination

Peptide Purity is determined by UV absorption at 220nm using RP-HPLC (reversed phase high performance liquid chromatography). This technique is used to separate, identify, and quantify components in a mixture, e.g. a target peptide and potential impurities.

• Peptide sample in solution is pumped through a adsorbent column
• Sample components interact differently with the adsorbent
• Different flow rates lead to separation of components
• UV detector generates a signal for each component at the end of the column
• Allows for quantitative analysis of components

Material requisition: > 150 µg/sample

Time needed: 1-2 days