When applying peptides in cellular assays or other biological systems, testing peptide samples for cell toxicity may be advisable as certain counter-ions or residual solvents are able to display cytotoxic properties. An in vitro toxicity test determines if the dissolved peptides show cytotoxic effects i.e. influence cellular metabolic activity. Therefore, the dye resazurin is used that is metabolized by living cells.
- A T cell lymphoma cell line is treated with the dissolved peptides
- After 24h the dye resazurin is added
- Viable, metabolically active cells will reduce the dye to resorufin
- Resofurin is photometrically quantified thus determining any cytotoxic effects of the peptides
Material requisition: 1 mg
Time needed: 2 days