Amino Acid Composition

The amino acid composition provides additional evidence on the assembly success for a given peptide. To confirm the results of peptide sequencing it is possible to perform a quantitative amino acid analysis. It provides quantitative information on amino acid ratios within a peptide and yields exact peptide content for accurate calculation of the peptide concentration.

•  Acidic hydrolysis of the peptide, transforming the sample into free amino acids
• Amino acids are quantified by ion exchange chromatography (or ion chromatography)
• Ion chromatography separates ionizable molecules based on their total charge and enables separation of similar molecules that would otherwise be difficult to separate

Material requisition: 1 mg/sample

Time needed: 2 weeks