Amino Acid Composition
The amino acid composition provides additional evidence on the assembly success for a given peptide. To confirm the results of peptide sequencing it is possible to perform a quantitative amino acid analysis. It provides quantitative information on amino acid ratios within a peptide and yields exact peptide content for accurate calculation of the peptide concentration.
- Acidic hydrolysis of the peptide, transforming the sample into free amino acids
- Amino acids are quantified by ion exchange chromatography (or ion chromatography)
- Ion chromatography separates ionizable molecules based on their total charge and enables separation of similar molecules that would otherwise be difficult to separate
Material requisition: 1 mg/sample
Time needed: 2 weeks