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FAQ: Single Peptides

Please refer to the corresponding frequently asked questions (FAQs) below.
If you can not find the question you are looking for, please contact our customer support team: peptide@jpt.com

How will Single Peptides be delivered?

Peptides are delivered freeze dried at room temperature.

How does JPT Peptide Technologies QC its products?

All synthetic peptides are analyzed by HPLC and MS. Besides MALDI-MS, our routine application involves HPLC-MS techniques to guarantee that a peak visible in the HPLC profile corresponds to the target mass.
Since peptides show different ion response depending on the actual primary sequence, availability of different MS-equipment is crucial for doubtless analysis.
JPT Peptide Technologies' state of the art equipment (MALDI-MS, HPLC-(ESI)MS (ion trap and quadrupole) guarantees unambiguous analysis.

How do I dissolve my peptides?

Solubility of peptides greatly depends on various parameters like primary and secondary structure, nature of modifications made, solvent, pH value and final concentration.
Therefore, solubility is hard to predict.
If a peptide is difficult to solubilize in aqueous solution, sonification will support solubility.
For basic peptides, 10% acetic acid has been recommended whereas for acidic peptides, aqueous ammonia or 10% ammonium bicarbonate solutions were shown to be helpful.
For peptides having poor solubility in aqueous solutions, organic solvents have to be applied.
Use the minimal amount of organic solvent (DMSO is preferred but DMF, DMA, isopropanol, methanol and others can also be used) to dissolve your peptide.
We strongly recommend dissolving the peptide first in the organic solvent.
In a second step, water or other aqueous media should be added in stepwise manner until the final concentration is reached.

What are the best storage conditions and how stable are the peptides?

Generally, most peptides are stable over years when stored at -20°C in freeze dried state.
However, peptides containing amino acids such as Met, Cys or N-terminal Gln tend to oxidation or pyroglutamate formation.
Since kinetics of such deterioration depends on primary peptide structure, stability can hardly be predicted.
Therefore, we recommend usage of peptides within 6 months. Recommended storage conditions are: -20°C or colder in a dry environment.
Avoid repeated freeze-thaw cycles.
Avoid long-term storage of aliquots in solution.
If solubilized aliquots can´t be avoided, store at -20°C and use sterile buffers at slightly acidic pH.

What to do if my peptide of interest is not available in JPT´s list?

Simply send us your peptide sequence, needed amount and purity to receive a quote on your custom peptide.
Contact: peptide@jpt.com

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