FAQ: Enzyme Profiling - Substrate Sets (Peptide Collections)
Please refer to the corresponding frequently asked questions (FAQs) below.
If you can not find the question you are looking for, please contact our customer support team: peptide@jpt.com
PepMix™ Peptide Pools
Pools of overlapping peptides from antigenic proteins for T-cell stimulation, T-cell expansion in T-cell assays such as ELISPOT, ICS or Flow Cytometry, e.g. for immune monitoring.
Transduction Enhancer
Protransduzin™ the highly efficient transduction enhancer increases viral gene transfer in pre-clinical and clinical applications, such as ex vivo gene therapy and CAR-T cell development.
Antigen Peptides
Antigen peptides represent specific epitopes for stimulation of T cells in T cell assays such as ELISPOT. We offer the corresponding MHC multimer for each antigen peptide. Antigens from different pathogens are available as well as tumor associated antigens.
MHC Multimers
MHC Multimers for reproducible detection, enumeration and isolation of antigen-specific T cells with disease specific peptides.
Proteomics Peptides & Kits
Peptide sets and pools, as well as assay standardization kits are available with stable isotope labeled or unlabeled proteotypic peptides for mass-spectrometry based proteomics such as MRM assays.
RepliTope™ Peptide Microarrays
Peptide microarrays that display overlapping peptide scans through antigens from infectious organisms or tumor associated antigens for antibody or serum profiling.
Chelate Peptides (DOTA)
DOTA is linked to molecules that have affinity for various structures (e.g. somatostatin receptors in neuroendocrine tumors). The resulting compounds can be bound to radionuclided and are used with a number of radioisotopes in cancer therapy and diagnosis
Cosmetic Peptides
Cosmetic Peptides such as Lysine and Cysteine Peptide are used for DPRA (Direct Peptide Reactivity Assay) for Skin Sensitization Testing. The DPRA measures the reaction of a chemical with synthetic peptides containing Cysteine (Ac‑RFAACAA‑COOH) or Lysine (Ac‑RFAAKAA‑COOH) to assess its sensitization potency. For research use only!
Abeta & Prion Peptides
Purified, fully analyzed, high quality peptides, synthesized from the peptide experts for Alzheimers and prion research.
Assay Services
We offer our extensive know-how and experience in peptide microarray incubation & data evaluation as full-service packages for many applications.
Enzyme Substrates
Large collections of peptidic enzyme substrates in microtiter plates or displayed on microarrays for screening and enyzme profiling.
Histone Peptide Tools
Our histone peptide library is the largest on the market and available as Peptide ELISA, Peptide Microarray or sets of histone peptides
JPT has a work force of >100 highly trained and skilled employees. 30 of our co-workers hold a university degree and 20 have a PhD in natural sciences.
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Please refer to the corresponding frequently asked questions (FAQs) below.
If you can not find the question you are looking for, please contact our customer support team: peptide@jpt.com
JPT Peptide Technologies provides a detailed protocol together with every Enzyme Substrate Set including plate layout and sequence list in Excel-format.
The protocols can also be downloaded from our website.
The peptides used in the Kinase Substrate Sets are N-terminally biotinylated and C-terminally amidated.
The peptides used in the Phosphatase Substrate Sets are N-terminally free and C-terminally amidated.
The peptides used in the Protease Substrate Sets carry Fluorophore and Quencher on N- and C-terminal ends respectively.
The enzyme concentration is mainly depending on the specific activity of your enzyme.
If you already have experimental data on you enzyme please use these as orientation.
If there is no information on enzyme activity available, we recommend to start with a high concentration and adjust later if necessary.
Peptide quantities are optimized for a single use.
All kinase substrates are available in larger quantities and can be purchased off-the-shelf.
For phosphatase and protease substrates customized synthesis is necessary and can be ordered fast and economically.
Please contact our customer support at peptide@jpt.com.
Generally, most peptides are stable over years when stored at -20°C in freeze dried state.
However, peptides containing amino acids such as Met, Cys or N-terminal Gln tend to oxidation or pyroglutamate formation.
Since kinetics of such deterioration depends on primary peptide structure, stability can hardly be predicted.
Therefore, we recommend usage of peptide sets within 6 months.
Once thawed and dissolved in buffer solution, JPT recommends performing the assay without further delay to avoid degradation.
The plates are shipped freeze dried at room temperature.
However, for long-term storage, JPT Peptide Technologies recommends storage at -20°C.
For Kinase Substrate Sets, the kinase in question, radioisotopic labeled ATP, as well as assay buffer are needed.
If fluorescence read-out should be performed, a suitable fluorescently labeled antibody or phosphospecific stain is needed.
For Phosphatase Substrate Sets, the phosphatase in question and the appropriate assay buffer are needed.
As read-out reagent JPT peptide Technologies recommends "Biomol green". For more details, please refer to the protocol.
For Protease Substrate Set, the protease in question and the appropriate assay buffer are needed.
For radioisotobic read-out of the kinase substrate set, a Streptavidin membrane (Promega, SAM-membrane) is needed, as well as a system for detecting incorporated radioisotopic labeled Phosphate (Phospho-Imager).
For the Phosphatase Substrate Sets, a microtiterplate-reader capable of detecting absorbance at 620-660nm is needed.
For the Protease Substrate Sets, a microtiterplate-reader capable of detecting fluorescence (Ex: 350nm, Em: 490nm). Preferred read-out procedure is from top but microtiterplates with clear bottom are also available.
Contact us:
JPT Peptide Technologies GmbH
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12489 Berlin, Germany
Technical Support:
T: +49-30-6392-7878
X: +49-30-6392-7888
e-mail: peptide@jpt.com
USA & Canada:
T: 1.888.JPT.COM0 (1.888.578.2660)
X: 1.888.JPT.COM6 (1.888.578.2666)
e-mail: peptide@jpt.com