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FAQ: Enzyme Profiling - Microarrays

Please refer to the corresponding frequently asked questions (FAQs) below.
If you can not find the question you are looking for, please contact our customer support team: peptide@jpt.com

Do you have a specific protocol used for the application of Enzyme Profiling microarrays?

JPT Peptide Technologies provides a detailed protocol together with every Enzyme Profiling microarray including slide layout and sequence list in a .gal-file format.
The protocols can also be downloaded from our website.

How are the microarrays shipped and how should the microarrays be stored upon arrival?

The microarray slides are shipped at room temperature.
For long-term storage, JPT Peptide Technologies recommends storage at +4°C.

What is the chemical stability of the microarrays / immobilized peptides?

As long as the microarrays are kept dry and cool (+4°C), the microarrays are stable for 18 months from date of delivery.

Can the microarrays be used again?

JPT Peptide Technologies recommends using the microarrays only once.

Do I need special reagents/chemicals to perform the assay?

For Kinase Profiling microarrays, the kinase in question, radioisotopic labeled ATP as well as assay buffer are needed.
If fluorescence read-out should be performed, a suitable fluorescently labeled antibody or phosphospecifc stain is needed.

For Phosphatase Substrate Sets, the phosphatase in question and the appropriate assay buffer are needed.
The read-out of the incorporated or cleaved phosphate is performed using anti-pTyr antibodies (JPT recommends Cell Signalling anti-pTyr-100 followed by Pierce anti-mouse-Dylight649).

For Protease Substrate Set, the protease in question and the appropriate assay buffer are needed.
The read-out of the full-length or cleaved peptides is performed using anti-pTyr antibodies (JPT recommends Cell Signalling anti-pTyr-100 followed by Pierce anti-mouse-Dylight649)

What hardware is needed for the read-out of the Enzyme Substrate Sets?

For radioisotopic read-out of the kinase profiling microarrays, a system for detecting incorporated phosphate is needed.
JPT recommends using a phosphoimaging screen.
If a fluorescence read-out is performed, a microarray scanner with appropriate wave-length and laser settings is needed.
For details, please refer to the protocol or contact our Customer support.
For read-out of the phosphatase or protease profiling microarrays, a microarray scanner with appropriate wave-length and laser settings is needed.
For details, please refer to the protocol or contact our Customer support (peptide@jpt.com).

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