FAQ: BioTides™
Please refer to the corresponding frequently asked questions (FAQs) below.
If you can not find the question you are looking for, please contact our customer support team: peptide@jpt.com
PepMix™ Peptide Pools
Pools of overlapping peptides from antigenic proteins for T-cell stimulation, T-cell expansion in T-cell assays such as ELISPOT, ICS or Flow Cytometry, e.g. for immune monitoring.
Transduction Enhancer
Protransduzin™ the highly efficient transduction enhancer increases viral gene transfer in pre-clinical and clinical applications, such as ex vivo gene therapy and CAR-T cell development.
Antigen Peptides
Antigen peptides represent specific epitopes for stimulation of T cells in T cell assays such as ELISPOT. We offer the corresponding MHC multimer for each antigen peptide. Antigens from different pathogens are available as well as tumor associated antigens.
MHC Multimers
MHC Multimers for reproducible detection, enumeration and isolation of antigen-specific T cells with disease specific peptides.
Proteomics Peptides & Kits
Peptide sets and pools, as well as assay standardization kits are available with stable isotope labeled or unlabeled proteotypic peptides for mass-spectrometry based proteomics such as MRM assays.
RepliTope™ Peptide Microarrays
Peptide microarrays that display overlapping peptide scans through antigens from infectious organisms or tumor associated antigens for antibody or serum profiling.
Chelate Peptides (DOTA)
DOTA is linked to molecules that have affinity for various structures (e.g. somatostatin receptors in neuroendocrine tumors). The resulting compounds can be bound to radionuclided and are used with a number of radioisotopes in cancer therapy and diagnosis
Cosmetic Peptides
Cosmetic Peptides such as Lysine and Cysteine Peptide are used for DPRA (Direct Peptide Reactivity Assay) for Skin Sensitization Testing. The DPRA measures the reaction of a chemical with synthetic peptides containing Cysteine (Ac‑RFAACAA‑COOH) or Lysine (Ac‑RFAAKAA‑COOH) to assess its sensitization potency. For research use only!
Abeta & Prion Peptides
Purified, fully analyzed, high quality peptides, synthesized from the peptide experts for Alzheimers and prion research.
Assay Services
We offer our extensive know-how and experience in peptide microarray incubation & data evaluation as full-service packages for many applications.
Enzyme Substrates
Large collections of peptidic enzyme substrates in microtiter plates or displayed on microarrays for screening and enyzme profiling.
Histone Peptide Tools
Our histone peptide library is the largest on the market and available as Peptide ELISA, Peptide Microarray or sets of histone peptides
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Please refer to the corresponding frequently asked questions (FAQs) below.
If you can not find the question you are looking for, please contact our customer support team: peptide@jpt.com
Biotides™ are the most inexpensive source of custom synthesized biotinylated peptides for screening application relying on the interaction between biotin and streptavidine.
approx. 50 nmol total peptide amount (approx. 10 nmol target peptide).
Up to 5000 BioTides™ will be delivered within 10 working days.
The Biotin is located at the N-terminal end of the peptide and is attached via a flexible linker system enabling optimal interaction with the streptavidine.
BioTides™ are synthesized on planar cellulose surfaces in a stepwise manner from the C- to N-terminus using preactivated Fmoc amino acids.
After synthesis, BioTides™ are cleaved from the membrane. This synthetical method is called "SPOT synthesis" and guarantees synthesis of high numbers of peptides in very short times.
Standard BioTides™ consist of an additional glycine acid or amide at the C-terminus due to technical reasons.
Individual C-terminal amino acids are also feasible at added costs (minimum order: 384 peptides).
All BioTides™ are synthesized as non-purified materials.
The purity of each BioTide™ depends on its primary sequence.
Typically the biotinylated peptide represents the main product.
Since purity of BioTides™ drops with increasing length we do not recommend BioTides longer than 20 amino acids.
5% of BioTides™ will be analyzed by LC-MS. In addition, JPT performs LC-MS of technical control sequences for each synthesis batch.
Additional LC-MS analyses is available upon request.
BioTides™ are delivered in ready-to-use in 96-well microplates (one freeze dried peptide per well, not immobilized, ready-to-dilute).
Minimum order size is 24 peptides.
For peptides with individual C-terminal amino acids minimum order size is 384 peptides.
Contact us:
JPT Peptide Technologies GmbH
Volmerstrasse 5
12489 Berlin, Germany
Technical Support:
T: +49-30-6392-7878
X: +49-30-6392-7888
e-mail: peptide@jpt.com
USA & Canada:
T: 1.888.JPT.COM0 (1.888.578.2660)
X: 1.888.JPT.COM6 (1.888.578.2666)
e-mail: peptide@jpt.com