Alanine scanning libraries are used to
- Identify key residues in epitopes
- Search binding parameters of enzymes
- Study binding sites of proteins
Creating an alanine scanning library, alanine systematically substitutes the residues at each amino acid position of the original peptide i.e. identified epitope (= alanine walk). Substitution of an important or essential amino acid by alanine results in less or no binding or activity.
Use our free and easy-to-use PepSequencer for generation of alanie scanning libraries!
Back to main Peptide Library Design page