Collection of 51stable isotope-labeled and absolutely quantified proteotypic peptides for 24 enzymes critically involved in the human central energy metabolism for direct usage in mass-spectrometry based proteomics (MRM)
Are you interested in your custom peptide libraries for your proteomics assays? Choose your sequence, amount and label. We will assist you along the way. Custom SpikeTides Reference Peptides.
JPT's heavy labeled and quantified SpikeTides™ Sets
JPT produced a set of stable isotope labeled proteotypic peptides for enzymes involved in energy metabolism. The peptides of this set are absolutely quantified for usage as internal standards in mass spectrometry assays by using our proprietary Qtag.
Benefits of JPT's heavy labeled & quantified SpikeTides™ Sets
- Absolute quantification of multiple proteins from a single sample
- First economic absolutely quantified peptide set available
- Multiplexed analysis of disease status and therapeutic success
- MRM transitions included
References:
Read References with Absolute Quantified Peptides - SpikeTides™ TQL & SpikeTides™ TQL PLUS
Application Note
Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides
Bindel et al., Application Note (2015) Full text
Testimonials
"My unit at the Banting and Best Department of Medical Research, University of Toronto Centre for Cellular and Biomolecular Research (CCBR) works on the systematic identification and quantification of proteins and protein complexes on a global level. In addition we have started to perform targeted proteomics to study protein regulation within pathways, e.g. signaling pathways during stem cell fate decision. For this we used JPT's various SpikeTides™ peptide products to build LC-MRM assays in a fast and cost efficient manner. More recently we also found new ways of using low cost SpikeTides™_TQL as quantitative peptide standards in order to quantify protein targets more accurately. We are currently in the process of validating this technology for the accurate quantification of various predicted microRNA targets."
Prof. Andrew Emili, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
"My group studies the proteomic composition of distinct chromatin domains, the mechanisms that operate to maintain the composition of histone modifications and the associated proteins. For precise and accurate identification and quantification of histone peptides that carry multiple post-translational modifications directly from biological samples JPT's SpikeTides™ _TQL peptide standards proved to be of excellent value for our research in various projects."
Prof. Dr. Axel Imhof, Adolf-Butenandt Institute, University of Munich, Germany
| Properties | Values |
|---|---|
| Application: | Proteomics |
| Category: | Absolute Quantified Peptides -SpikeTides™ TQL |
| Layout: | Freeze-dried in 1 x 96 tube rack |
| Organism: | Human |
| Protein Name: | Metabolic Enzymes |
| Purity: | crude |
| Quantification: | Yes |
| Information | Values |
|---|---|
| Sequence: | Details upon request |
| Specifications: | Absolutely quantified proteotypic stable isotope-labeled peptides with Qtag |
All production is ISO 9001:2015 certified.
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