SpikeMix™ Wnt Signaling Pathway (Human) - heavy



Product Description

Pool of of 343 representative proteotypic peptides (including 67 natural sequence variants and 36 peptides with annotated modifications) for 65 proteins of the human Wnt pathway for direct usage in mass-spectrometry based proteomics (MRM).

Amount: 10 pmol/peptide
Purity: crude
Delivery Format: Freeze-dried in plastic vial
Application(s): Mass spectrometry, Proteomics
Indication(s)/Topic(s): Breast cancer, Cancer, Gastric cancer, Melanoma
Delivery Time: 2-5 days

JPT's SpikeMix™
JPT produces specific mixtures of stable isotope-labeled peptides for the detection of relevant proteins by mass-spectrometry based proteomics. The mixed peptides are available as stable isotope labeled peptides (using heavy Arg U-13C6, U-15N4 or Lys U-13C6, U-15N2).

Benefits of JPT's SpikeMix™
- Quantification of multiple proteins from a single sample
- Economic premixed heavy peptide sets available for different species
- Multiplexed analysis of disease status and therapeutic success


References for SpikeMix™ Wnt Signaling Pathway (Human) - heavy

Read References with Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™

Application Notes
Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens
Soderblom et al., Application Note (2015) Full text
Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides
Bindel et al., Application Note (2015) Full text
Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides
Colangelo, Application Note (2014) Full text

"As Director of the Protein Profiling at Yale Keck Biotechnology Resource Facility, I coordinated a collaboration between the Association of Biomolecular Resource Facilities (ABRF) standard proteomic research group (sPRG) and JPT Peptide Technologies to develop the ABRF cross-species SpikeMix™ peptide pool. The joint development turned out to be an extraordinary effective endeavor and the resulting product was qualified in more than 52 proteomics labs around the globe. I was impressed by JPT's scientific and technological capabilities as well as their enthusiasm to drive the project forward."
Christopher M. Colangelo, Director of Protein Profiling at Keck Biotechnology Resource Facility, Yale University, USA

"Our research at Stanford University seeks to discover unconventional translation products, and proteins made by unsequenced organisms; to characterize a wide array of protein post-translational modifications; and to perform peptide antigen binding assays. On all of these fronts, we are in need of high quality peptide standards for assay development and protein quantification. The successful collaboration with the team at JPT Peptide Technologies and their wide range of products has been a critical factor for advancing my group's research."
Joshua E. Elias, Ph.D., Assistant Professor, Chemical & Systems Biology, Stanford University School of Medicine, Clark Center, Stanford, CA


Documentation for SpikeMix™ Wnt Signaling Pathway (Human) - heavy


Properties of SpikeMix™ Wnt Signaling Pathway (Human) - heavy

Properties Values
Application: Mass spectrometry, Proteomics
Category: SpikeMix™ for Targeted Proteomics
Indication / Topic: Breast cancer, Cancer, Gastric cancer, Melanoma
Organism: Homo sapiens (Human)
Protein Name: Selected proteins

Further Information to SpikeMix™ Wnt Signaling Pathway (Human) - heavy

Information Values
Sequence: See Layout and Sequences under Product Documentation
Specifications: Mix of 343 stable isotope labeled peptides from proteins of the human Wnt pathway.
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Health - made in Germany
Quality Assurance

All production is performed according to ISO 9001:2015 standards.